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Rosette formation of pig peripheral blood lymphocytes with sheep red blood cells.
Authors:E Kaupp  R Pabst  F Trepel
Affiliation:1. Dept. of Psychological Sciences, University of Connecticut, Storrs, CT, USA;2. Haskins Laboratories, New Haven, CT, USA;3. Dept. of Radiology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada;4. Dept. of Educational Psychology, Texas A&M University, College Station, TX, USA;5. Centre for Cognitive Neuroscience & Department of Psychology, University of Salzburg, Salzburg, Austria;1. School of Science and Technology, Meiji University, Kawasaki 214-8571, Japan;2. Graduate School of Advanced Integration Science, Chiba University, Chiba 263-8522, Japan;3. Center for Organic Photonics and Electronics Research, Kyushu University, Fukuoka 819-0395, Japan;4. International Institute for Carbon Neutral Energy Research (WPI-I2CNER), Kyushu University, Fukuoka 819-0395, Japan;5. Center for Frontier Science, Chiba University, Chiba 263-8522, Japan
Abstract:
Peripheral blood lymphocytes of normal young pigs formed spontaneous and EAC rosettes with sheep red blood cells. Pretreatment of the SRBC with a sulphydryl reagent increased the percentage of spontaneous rosettes and the number of SRBC bound by the lymphocytes. The influence of an incubation period prior to centrifugation and the duration and temperature of the following incubation were tested for spontaneous and EAC rosettes. The importance is stressed to define the number of bound SRBC to call a lymphocyte a "rosette-forming cell". Rosette formation is interpreted as a quantitative rather than a qualitative marker.
Keywords:
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