α-淀粉酶抑制剂筛选方法的优化

目的 对α-淀粉酶活性测定方法进行优化，用于α-淀粉酶抑制剂的筛选。方法 采用3,5-二硝基水杨酸方法（Bernfeld法）测定α-淀粉酶活性。本实验对反应体系的总体积、可溶性淀粉浓度、α-淀粉酶浓度、阳性药阿卡波糖浓度分别进行考察和优化。结果 建立一个系统稳定、结果可靠、重现性好的α-淀粉酶抑制剂筛选方法。结论 优化后的Bernfeld法可用于α-淀粉酶抑制剂的筛选。

Optimization of the bioassay method for screening of α-amylase inhibitors

Objective To optimize the determining method of α-amylase activity for screening the inhibitors of α-amylase. Methods To determine the α-amylase activity by 3,5-dinitrosalicylic acid colorimetric assays (Bernfeld method), and to investigate and optimize the volume of reactive system, the contents of starch, α-amylase and acarbose (standard drug). Results The optimized method was stable, reliable and well repeated. Conclusion The optimized method can be used to screen the inhibitors of α-amylase.