高原肺水肿大鼠模型的建立与研究

 目的 模拟高原环境,研究大鼠急性低氧复合运动高原肺水肿的发生及低氧习服后的改变.方法 健康SD-大鼠分为常氧对照组(n=10)、5 000 m急性低氧组(n=10)及3 000 m低氧习服组(n=10).对比研究大鼠的肺血流动力学、肺水肿程度及肺组织病理形态学改变.结果 与常氧对照组相比,急性低氧组大鼠动脉血氧分压及氧饱合度显著降低,肺体指数及肺含水率升高,病理学显示肺间质充血,肺泡隔增宽等间质性肺水肿表现.低氧习服组明显改善.结论 成功建立高原间质性肺水肿模型及低氧习服模型,有利于进一步研究高原肺水肿低氧习服机制.

Development and study of rat model of high altitude pulmonary edema

Objective To study the effects of acute hypoxia combined with exercise and hypoxic preconditioning on the development of high altitude pulmonary edema. Methods Healthy male and female SD rats （ n = 30） were randomly divided into 3 groups： normal control group （ n = 10）, acute hypoxic group （ n = 10, at 5 000 m） and 3 000 m hypoxic preconditioning group （ n = 10）. The pulmonary arterial pressure was observed and pulmonary tissue was collected for pathological study. Results PaO-2 and SaO-2 in acute hypoxic group decreased signifieuntly. Water ratio of lung and lung - body weight index increased significantly. Pulmonary interstitial edema was found in rats exposed to simulated altitude of 5 000 m for 24 hours. Hypoxic preconditioning relieved severity of pulmonary edema. Conclusions We successfully developed the model of high altitude pulmonary interstitial edema by exposed SD rats to simulated altitude of 5000m for 24 hours and hypoxic preconditioning model.