| 大鼠“曲泽”,“大陵”和“中冲”穴区之间神经血管显微结构的比较 |
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| 引用本文： | 张知云,徐东升,张建梁,王佳,崔晶晶,景向红,白万柱.大鼠“曲泽”,“大陵”和“中冲”穴区之间神经血管显微结构的比较[J].世界针灸杂志,2020,30(1):61-64. |
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| 作者姓名： | 张知云 徐东升 张建梁 王佳 崔晶晶 景向红 白万柱 |
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| 作者单位： | 中国中医科学院针灸研究所,北京100700,中国;浙江中医药大学第三临床医学院,杭州310053,中国;中国中医科学院针灸研究所,北京100700,中国 |
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| 摘 要： |
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Comparison of the neurovascular microstructure in local skin tissues at “PC3” “PC7” and “PC9” regions in the rat 大鼠“曲泽”,“大陵”和“中冲”穴区之间神经血管显微结构的比较 |
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| Institution: | 1. Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China (中国中医科学院针灸研究所, 北京 100700, 中国);2. The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China (浙江中医药大学第三临床医学院, 杭州 310053, 中国);2. Department of Animal Science, University of California Davis 95616;3. January Innovation Inc. 5750 East Harney Lane, Lodi CA 95240;1. Henan University of Chinese Medicine, Zhengzhou 450046, China (河南中医药大学, 郑州 450046, 中国);2. Henan Provincial People''s Hospital, Zhengzhou 450008, China (河南省人民医院, 郑州 450008, 中国);3. Shenzhen Pingle Orthopaedic Hospital, Shenzhen 518122, Guangdong, China (深圳平乐骨伤科医院, 广东深圳 518122, 中国);4. Research Institute of Acupuncture and Moxibustion of Henan University of Chinese Medicine, Zhengzhou 450008, China (河南中医药大学针灸研究所, 郑州 450000, 中国);1. Orthopedics and Traumatology Department of Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, China (上海市中医医院骨伤科, 上海200071, 中国);2. School of Rehabilitation Science, Shanghai University of TCM, Shanghai 201203, China. (上海中医药大学康复医学院, 上海 201203, 中国);3. Acupuncture and Tuina College, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China (上海中医药大学针灸推拿学院, 上海 201203, 中国) |
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| Abstract: | ObjectiveThe aim of this study was to compare the microstructure of blood vessels and nerve fibers between different acupoints in the rat skin from the perspective of histology.MethodsThe skin tissue at “Qūzé” (曲泽 PC3)” “Dàlíng (大陵 PC7)” and “Zhōngchōng (中冲 PC9)” regions was taken from the rat forelimb along the pericardium meridian, and cut sagittally with a freezing microtome. After that, the skin sections were stained by fluorescent histochemistry and immunohistochemistry with phalloidin and calcitonin gene-related peptide (CGRP) to reveal the microstructure of blood vessels and nerve fibers respectively, and then examined under a laser scanning confocal microscope.ResultsThe microstructure of blood vessels and nerve fibers was clearly labeled with phalloidin and CGRP, respectively. The blood vessels and nerve fibers were observed in the dermis of “PC3” “PC7” and “PC9” regions, where the bundle of nerve fibers distribute in parallel to the blood vessels, and sent out thin branches to surround the wall of the blood vessels. In contrast, the density of blood vessels and nerve fibers in “PC3” “PC7” and “PC9” were presented orderly in an increased tendency.ConclusionThe results of present study demonstrate the differences of the neurovascular microstructure among “PC3” “PC7” and “PC9” in the rat, providing a histological view to insight into the specificity of different acupoints. |
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