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| 乙肝患者外周血单个核细胞FOXP3 mRNA表达检测方法的建立及应用 | |
| 引用本文： | 郭凯,黄长形. 乙肝患者外周血单个核细胞FOXP3 mRNA表达检测方法的建立及应用[J]. 医学研究生学报, 2008, 21(11) |
| 作者姓名： | 郭凯 黄长形 |
| 作者单位： | 1. 第四军医大学唐都医院全军感染病诊疗中心,陕西西安,710038;中国人民解放军第161中心医院,湖北武汉,430015 2. 第四军医大学唐都医院全军感染病诊疗中心,陕西西安,710038 |
| 基金项目： | 第四军医大学2003年度后备人才基金资助计划项目 |
| 摘 要： | 目的:比较乙肝患者与健康人外周血单个核细胞(PBMC)中 FOXP3 mRNA表达水平差异. 方法:利用实时荧光定量RT-PCR的方法检测25例慢性乙肝患者以及11名健康人PBMC中FOXP3 mRNA的水平. 结果:乙肝患者PBMC中FOXP3 mRNA的表达水平高于健康对照组,差异具有统计学意义(P＜0.01),且与血清中的病毒含量呈正相关(r=0.70,P＜0.01),而与谷丙转氨酶(ALT)水平无关(r=0.06,P＞0.05). 结论:慢性乙肝患者FOXP3表达水平可能是HBV感染持续的一个因素. |
| 关 键 词： | 乙肝 调节性T细胞 |
Real-time RT-PCR for detection of FOXP3 expression in the peripheral blood mononuclear cells of hepatitis B patients |
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| GUO Kai,HUANG Chang-xing. Real-time RT-PCR for detection of FOXP3 expression in the peripheral blood mononuclear cells of hepatitis B patients[J]. Bulletin of Medical Postgraduate, 2008, 21(11) | |
| Authors: | GUO Kai HUANG Chang-xing |
| Affiliation: | GUO Kai1,2,HUANG Chang-xing1 (1.PLA Center for Diagnosis , Treatment of Infectious Diseases,Tangdu Hospital,the Fourth Military Medical University,Xi'an 710038,Shaanxi,China,2.The 161st Hospital of PLA,Wuhan 430015,Hubei,China) |
| Abstract: | Objective: To compare the expression levels of FOXP3 mRNA in the peripheral blood mononuclear cells(PBMC) of hepatitis B patients and healthy persons.Methods: Real-time fluorescence relative quantitative RT-PCR was used to determine the FOXP3 mRNA levels in the PBMCs from 25 patients with chronic hepatitis B and 11 healthy subjects.Results:The FOXP3 mRNA levels were significantly higher in the hepatitis B patients than in the normal subjects(P<0.01),with a positive correlation with the HBV load(r=0.70,P<0.0... |
| Keywords: | FOXP3 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |