端粒酶逆转录酶和c-myc基因在子宫内膜样癌及子宫内膜增生中的表达

目的　探讨端粒酶逆转录酶 (hTERT)及c myc基因在子宫内膜增生及癌变过程中的作用、意义及二者相关性。方法　所用标本包括 14例子宫内膜单纯增生 ,8例复合增生 ,10例不典型增生 ,42例内膜样癌 ,用原位杂交法检测hTERT和c mycmRNA表达。结果　 (1)hTERT在子宫内膜单纯、复合、不典型增生病变和内膜样癌中阳性结果分别 2 / 14、4/ 8、8/ 10和 92 9% (3 9/ 42 ) ,前两组均为弱阳性表达 ,后两组多为中度和强阳性 ,统计分析表明不典型增生病变和内膜样癌中hTERT表达高于单纯和复合增生 (P <0 0 5)。c myc在子宫内膜单纯、复合、不典型增生病变和内膜样癌中阳性结果分别 3 / 14、1/ 8、5/ 10和 54 8% (2 3 / 42 ) ,后两组c myc阳性率显著高于前两组 (P <0 0 5) ;不典型增生病变的c myc阳性水平高于单纯及复合增生 (P <0 0 5)。(2 )hTERT阳性水平与内膜样癌分化相关(P <0 15) ;c myc阳性率随内膜样癌浸润深度增加而递增 (P <0 0 5)。 (3 )子宫内膜增生和内膜样癌各组中hTERT与c myc表达均不相关 (P >0 0 5)。结论　hTERT及c myc基因过表达与子宫内膜不典型增生及恶性转化相关 ,并与内膜样癌演进以及不良预后有关 ,但其两者表达之间无相关性

Expression of human telomerase reverse transcriptase and c-myc transcripts in endometrial carcinoma and its precursors

ObjectiveTo study the possible role of hTERT and c-my c in endometrial carcinogenesis. MethodsThe expression of hTERT and c-myc mRNA was examined by in situ hybridization of endometrial samples from 14 cases with simple hyperplasia, 10 with complex hyperplasia, 8 with atypical hyperplasia and 42 with endometrio id carcinoma. ResultsExpression of hTERT was demonstrated in s amples with simple hyperplasia, complex hyperplasia,atypical hyperplasia and carcinoma at frequencies of 2/14,4/8, 8/10 and 39/42(92.9%), respectively. The prevalence and intensity of the hTERT signal was greater in the carcinomas and lesions with atypical hyperplasia than those with simple or complex hyperplasia (P<0.05). The expression of c-myc was demonstrated in samples with simple hyperplasia, complex hyperplasia,atypical hyperlasia and carcinoma at frequencies of 3/14, 1/8, 5/10 and 23/42(54.8%), respectively. The frequency of c-myc expression was higher in carcinomas and hyperplastic lesions with atypia than those in lesions with simple or complex hyperplasia without atypia (P<0.05). The expression of hTERT was shown to be correlated with the level of differentiation (P<0.05), while the c-myc expression appeared to be associated with the depth of myometrial invasion (P<0.05). The expression levels of hTERT and c-myc were not found to be correlated with each other in the tissues examined(P>0 05). ConclusionsThe expression of hTERT and c-myc may be involved in the progression from the endometrial aypical hyperplasia to invasive carcionoma. The correlation between hTERT and c-myc in end ometrial hyperplasiaand carcinoma are not found.