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| 麻黄中挥发性化学成分的顶空固相微萃取技术气相色谱/质谱分析 | |
| 引用本文： | 黄滔敏,陈念祖,杨蓓,段更利.麻黄中挥发性化学成分的顶空固相微萃取技术气相色谱/质谱分析[J].复旦学报(医学版),2006,33(6):848-850. |
| 作者姓名： | 黄滔敏 陈念祖 杨蓓 段更利 |
| 作者单位： | 1. 复旦大学药学院药物分析教研室,上海,200032;复旦大学附属眼耳鼻喉科医院药剂科,上海,200031 2. 复旦大学附属眼耳鼻喉科医院药剂科,上海,200031 3. 复旦大学药学院药物分析教研室,上海,200032 |
| 摘 要： | 目的 采用顶空固相微萃取技术（HS-SPME）和GC／MS分析麻黄中挥发性化学成分。方法 用固相微萃取技术对麻黄中挥发性化学成分进行顶空取样，用GC／MS对其进行定性分析，并与传统的水蒸气蒸馏（SD）方法进行比较。结果 固相微萃取法30min可萃取出20个挥发性化学成分，水蒸气蒸馏法蒸馏提取6h可萃取出24个挥发性化学成分。结论 顶空固相微萃取技术对麻黄中挥发物的提取分析，更简单、快速、灵敏和无溶剂，可以作为中药麻黄的质量评估方法之一。 |
| 关 键 词： | 麻黄 挥发成分 固相微萃取技术 气相色谱/质谱法 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006年4月10日 |
Analysis of volatile compounds in ephedrae herba by solid-phase microextraction followed by gas chromatography-mass spectrometry |
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| HUANG Tao-min,CHEN Nian-zhu,YANG Bei,DUAN Geng-li.Analysis of volatile compounds in ephedrae herba by solid-phase microextraction followed by gas chromatography-mass spectrometry[J].Fudan University Journal of Medical Sciences,2006,33(6):848-850. | |
| Authors: | HUANG Tao-min CHEN Nian-zhu YANG Bei DUAN Geng-li |
| Abstract: | Purpose To develop a headspace solid-phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry for the analysis of volatile compounds in ephedrae herba. Methods The SPME method for the extraction of volatile oil from ephedrae herba has been compared with the conventional steam distillation (SD). Results Twenty compounds were identified by using SPME after extraction for 30 min. Twenty-four volatile compounds were identified by the conventional SD after extracting for 6 h and using large amount of organic solvent. Conclusions The proposed SPME is a simple, rapid, solvent-free and superior method for the analysis of volatile compounds in ephedrae herba. |
| Keywords: | ephedrae herba volatile compounds solid-phase microextraction gas chromatog raphy-mass spectrometry |
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