检测乳腺癌组织内DNA倍体和ER含量的意义

本文应用流式细胞分析术检测了65例乳腺癌的细胞核 DNA 含量,同时用免疫组化 PAP 法测定肿瘤细胞中雌激素受体(Estrogen receptor ER)表达状况.结果表明二者呈负相关(P<0.01),ER(+)者异倍体占52.6%(20/38)。ER(-)者异倍体占85.2%(23/27)。提示乳腺癌的 ER 表达与 DNA 含量有一定的关系;异倍体的存在可能是 ER(+)患者对内分泌治疗不敏感的一个重要因素。

Significance of determination of DNA ploidy and ER status in breast cancer

The nucleic DNA content of 65 cases of breast cancer was determined by flow cytometry (FCM).At the same time the estrogen receptor(ER)status in breast cancer cell was assayed immunohistochemically(PAP).The results showed a negative correlationship between them (P<0.01).The aneuploid rate was 52.6%(20/38)in patients with ER(+),but 85.2%(23/27) in those with ER(-).This suggested a close correlationship between the DNA content and ER status in breast cancer.The presence of DNA aneuploidy might chiefly account for the non-responsiveness to endocrine therapy of breast cancer patients with ER(+).