消渴康对糠尿病肾病大鼠肾脏基质金属蛋白酶9与蛋白酶组织抑制剂1表达的影响

目的：观察消渴康对糖尿病肾病大鼠肾脏基质金属蛋白酶9（ matrix metalloproteinase-9,MMP-9）与金属蛋白酶组织抑制剂1（tissue inhibitor of metalloproteinases-1,TIMP-1）表达的影响。方法 SD大鼠单侧肾切除后2周,一次性腹腔注射链脲佐菌素（ streptozotocin,STZ）,72小时后血糖浓度≥16.7 mmol/L,尿糖定性〉＋＋＋,确定糖尿病肾病造模成功,随机分为模型、氯沙坦和消渴康大、中、小剂量治疗组,分别灌胃12周后,免疫组化法检测各组糖尿病肾病大鼠肾脏 MMP-9与TIMP-1表达情况。结果消渴康小剂量治疗组极显著增加糖尿病肾病大鼠肾组织MMP-9表达（ P〈0．01）,消渴康中、小剂量治疗组极显著降低糖尿病肾病大鼠肾组织TIMP-1表达（P〈0．01）。结论消渴康对糖尿病肾病大鼠肾脏异常细胞外基质（ extracellu-lar matrix,ECM）堆积具有一定促降解作用。

The affection of Xiaokekang on expression of MMP-9 and TIMP-1 in kidney of diabetic nephropa-thy rats

Objective To observe the affection of Xiaokekang on expression of MMP-9 and TIMP-1 in kidney of Diabetic Nephropathy（ DN） rats. Methods 2 weeks later after nephrectomy in one-side of SD rats, injected streptozotocin（ STZ） one-time by intraperitoneal injection, 72 hours later, if blood sugar concentration ≥16. 7 mmol/L and Glucose 〉 ＋ ＋ ＋, determined DN model was successful, then estab-lished the modele group, losartan group,xiaoda group, xiaozhong group, and xiaoxiao group at random, de-tected MMP-9 and TIMP-1 in kidney of DN rats after intragastric administration for 12 weeks. Results Expression of MMP-9 Extremely significantly reduced in kidney of xiaoxiao group （ P〈0. 01 ） and signifi-cantly increased in kidney of xiaoxiao group and xiaozhong group（P〈0. 01）. Conclusion There was cer-tain acceleration of degradation to abnormal accumulation ECM of kidney by Xiaokekang.