Quantitative autoradiography of 5-HT1D and 5-HT1E binding sites labelled by [H]5-HT, in frontal cortex and the hippocampal region of the human brain |
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Authors: | Pascal Barone Daniel Jordan Frdric Atger Nicolas Kopp Gilles Fillion |
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Institution: | Pascal Barone, Daniel Jordan, Frédéric Atger, Nicolas Kopp,Gilles Fillion, |
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Abstract: | In human cortex and hippocampus area, 3H]5-HT (5 nM) labels 5-HT1A, 5-HT1D and 5-HT1E sites. After masking 5-HT1A receptors by 0.1 μM 8-OH-DPAT, the binding displaced by 0.1 μM 5-CT presumably represented 5-HT1D sites and the remaining binding 5-HT1E sites. In frontal cortex, 5-HT1A receptors represented the main binding in layers II and VI and a lower fraction on other layers. 5-HT1D and 5-HT1E sites, were more homogeneously distributed in layers II to VI (21–34% of specific 3H]5-HT binding). 5-HT1E sites were of similar affinities (KD close to 6–8 nM) in the cortical layers II to VI. In CA1 field of hippocampus, (pyramidal layer, stratum radiatum, molecular layer), CA2 and dentate gyrus, 5-HT1A receptors represented the major fraction, 5-HT1D sites a significant fraction and 5-HT1E a minor fraction of the specific 3H]5-HT binding. In CA3–CA4 fields, 5-HT1A receptors were less densely present, 5-HT1D sites were predominant and 5-HT1E sites represented a significant fraction (27%). The highest densities of 5-HT1E sites have been measured in subiculum, where 5-HT1A, 5-HT1D, and 5-HT1E binding sites were equally represented and in entorhinal cortex where 5-HT1E sites represented the major binding in layer III. They were also present in layers II and IV (29 and 24%) and, to a lesser extent, in layers V and VI. 5-HT1A sites were predominant in layer VI, II and V and were less abundant in other layers. 5-HT1D were homogeneously present in layers II, III, IV and were present in low amounts in other layers. No 5-HT1E were detected in choroid plexus, where 3H]5-HT was dramatically reduced by mesulergine (5-HT2C receptors). No significant displacement of 3H]5-HT by mesulergine was measured in other structures. |
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Keywords: | Serotonin receptor Serotonin1D Serotonin1E Human brain Autoradiography Post-mortem [3H]SerotoninAuthor Keywords:5-CT 5-car☐ amidotryptamine 5-HT 5-hydroxytryptamin serotonin 8-OH-DPAT 8-hydroxy-2(di-n-propyl-amino) tetralin TFMPP trifluoromethylpiperazine |
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