正常精子体外与活性氧作用后超微结构观察

目的 :观察精子与活性氧 (ROS)作用后超微结构变化。　方法 :采用Percoll梯度离心法选择具有正常生理功能的精子作为正常精子模型 ,应用次黄嘌呤 黄嘌呤氧化酶体系产生ROS ;在有氧环境下ROS与精子模型孵育后 ,采用透射电镜观察精子超微结构。　结果 :正常精子模型与ROS作用后 ,精子膜及顶体存在不同程度的损伤 ,精子线粒体结构出现异常。　结论 :过多的ROS可致精子膜、顶体以及线粒体的超微结构改变 ,损害精子功能。

Ultrastructural Observation of Normal Spermatozoa Incubated with ROS in vitro

Objectives: To observe ultrastructural changes of spermatozoa after incubate with reactive oxygen species ( ROS ). Methods: Spermatozoa of normal physiological functions selected from semen samples by Percoll gradient centrifugation technique were regarded as normal sperm models in present study. Ultrastructural changes of spermatozoa observed by transmission electron microscope after model spermatozoa were incubated with ROS generated by hypoxanthine and xanthine oxidase under aerobic environment. Results: After model spermatozoa were incubated with ROS, impairment of various extent in membrane and acrosome of spermatozoa and abnormality in mitochondria of spermatozoa were found. Conclusions: Excessive ROS may cause ultrastructural change in membrane, acrosome and mitochondria of spermatozoa and impair function of spermatozoa.