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Bcl-2,Survivin,CD44V6在宫颈癌组织中的表达及其与HPV16/18感染的相关性分析
引用本文:赵富玺,穆雅琴,郭俊晨,刘润花. Bcl-2,Survivin,CD44V6在宫颈癌组织中的表达及其与HPV16/18感染的相关性分析[J]. 大同医学专科学校学报, 2006, 26(4): 1-5,15
作者姓名:赵富玺  穆雅琴  郭俊晨  刘润花
作者单位:山西大同大学医学院,037008;山西大同大学医学院,037008;山西大同大学医学院,037008;山西大同大学医学院,037008
摘    要:研究Bcl-1、Survivin、CD44V6在宫颈癌组织中的表达及其与细胞凋亡、HPV16/18感染的相关性,以探讨宫颈癌的发生机制,指导其诊断预后;方法:采用免疫组织化学方法对宫颈癌、宫颈上皮内瘤样病变(CIN)和正常宫颈组织的石蜡标本进行Bcl-2、Survivin、CD44V6的检测,同时采用TUNEL法和PCR技术检测细胞凋亡指数和HPV16/18感染情况;结果:Bcl-2、Sur—vivin、CD44V6在宫颈癌组织中的阳性率与CIN、正常宫颈组织有显著性差异,且随着临床分期病理分级的升高,阳性率差异有显著性,其表达率与淋巴结转移有关。HPV16/18在正常宫颈、CIN和宫颈癌组织中的阳性率逐渐升高,差异有显著性,但与临床分期病理分级淋巴转移无关。AI在宫颈癌中显著高于CIN和正常宫颈组织,差异有显著性,且其与宫颈癌的临床分期病理分级淋巴结转移有关。Bcl-2、Survivin和AI均与HPV16/18感染有相关性;结论:Bcl-2、Survivin、CD44V6在宫颈癌组织中的异常表达与HPV16/18感染有关,与宫颈癌的恶变程度有关,可作为筛查宫颈癌和判断预后的有利指标。

关 键 词:Bcl-2  Survivin  CD44V6  细胞凋亡  HPV  宫颈癌
收稿时间:2006-06-06
修稿时间:2006-06-06

Relationship between apoptosis with Bcl-2 and Survivin, CD44V6 and HPV16/18 infection in uterine cervical carcinogenesis
Zhao Fuxi,Mu Yaqin,Guo Juncheng,Liu Ronghua. Relationship between apoptosis with Bcl-2 and Survivin, CD44V6 and HPV16/18 infection in uterine cervical carcinogenesis[J]. Journal of Datong Medical College, 2006, 26(4): 1-5,15
Authors:Zhao Fuxi  Mu Yaqin  Guo Juncheng  Liu Ronghua
Affiliation:Zhao Fuxi Mu Yaqin Guo Juncheng Liu Ronghua
Abstract:Objective:To investigate the relationship between apoptosis with Bcl-2 and Survivin,CD_ 44 V_6 and HPV_ 16/18 infection in uterine cervical carcinogenesis.Method:Formalin-fixed,paraffin-embedded samples from cervical carcinomas,cervical intraepithelial neoplasia and normal cervical tissues were studied.Using streptavidin-biotin roxidase (s-p) immunohistochemical technique,the authors examined the expression of Bcl-2,Survivin and CD_ 44 V_6 in these samples.The number of apoptosis cells was assessed in situ by TdT-mediated dUTP-biotin end labeling (TUNEL) method.The infection of HPV type 16,18 DNA were determined by PCR.Result:There were significant differences for Bcl-2,Survivin,CD_ 44 V_6 and AI between carcinomas,CIN and normal cervices,respectively.Expression of Bcl-2,Survivin,CD_ 44 V_6 and AI were corrleated with tumor grades,clinical stages and lymph node metastasis.The positive rate of HPV_ 16/18 was significant difference between cervical carcinomas,CIN and nomal tissues and was not associated with tumor grades,clinical stages and lymph node metastasis.The infection of HPV_ 16/18 was associated with the expression of Bcl-2,Survivin and AI,respectively.Conclusion:The innormal expression of Bcl-2,Survivin and CD_ 44 V_6 are associated with the infection of HPV_ 16/18 .They possibilly become useful indexs for the primary screening and prognosis of cervical carcinomas.
Keywords:Bcl-2  Survivin  CD44V6  HPV
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