PON基因族多态性与散发性阿尔茨海默病的相关性研究

目的探讨对氧磷酶（paraoxonase,PON）基因多态性与散发性阿尔茨海默病（SAD）的相关性。方法用常规方法从被试者外周血细胞中提取基因组DNA,应用聚合酶链反应-限制性片断长度多态性的分析方法（PCR-RFLP）检测PON基因的多态性。被试者包括181例SAD患者和年龄、性别相匹配的正常对照149例。观察PON1基因Q192R多态性、PON2基因S311C多态性分布,用卡方检验和逻辑回归分析进行统计。结果PON1基因的Q192R多态性及PON2基因S311C多态性,其总体基因型频率及等位基因频率在SAD患者与对照者间无显著性差异（χ^2=0.086,P〉0.05;χ^2=0.883,P〉0.05）。但PON2基因S311C多态性在75岁以上高龄SAD患者与对照者之间基因型的分布有明显统计学意义（χ^2=5.009,P=0.025）。结论PON1基因Q192R多态性与SAD不存在关联,但PON2基因S311C多态性可能与高龄人群SAD有关联。

The association of polymorphism of PON gene in sporadic Alzheimer's disease

Objective To explore the association between polymorphism of the paraoxonase(PON)gene and sporadic Alzheimer's disease(SAD).Methods Genomic DNA was extracted from peripheral blood cells of patients and controls,by conventional means.Polymorphism distribution of the PON gene was detected by using polymerase chain reaction-restrictive fragment length polymorphism(PCR-RFLP).The subjects examined include 181 patients with SAD,along with age-and sex-matched 149 controls.The distributions of the Q192R polymorphis...