微阵列酶联免疫技术诊断肺结核的临床应用

目的评价微阵列酶联免疫技术(Array-ELISA)在肺结核诊断中的临床应用价值.方法 应用结核分枝杆菌微阵列蛋白芯片试剂盒检测76例肺结核患者、27例肺部其他疾病患者和80例健康体检者的血清标本.结果 ArrayELISA诊断肺结核的敏感性、特异性、准确度、阳性预测值及阴性预测值分别为76.32％、97.20％、93.64％、95.08％和85.25％.肺结核组各单项抗体指标检测的阳性率高于干扰组和健康对照组,差异有统计学意义(P＜0.05)；4种抗体联合检测的敏感性、准确度和阴性预测值均高于单项抗体检测指标,差异有统计学意义(P＜ 0.05),特异性和阳性预测值二者差异无统计学意义(P＞0.05).结论 4种抗体联合检测的敏感性和准确度高,Array-ELISA技术操作简便快速,是辅助诊断肺结核的有效方法.

Clinical application of Array-ELISA for diagnosis of pulmonary tuberculosis

OBJECTIVE To evaluate the value of clinical application of Array-ELISA for diagnosis of pulmonary tuberculosis(TB).METHODS TB Array-ELISA was used to detect serum samples of 76 TB patients,27 non-TB lung disease patients and 80 healthy subjects.RESULTS The sensitivity,specificity and precision of the technology for detection of TB by Array-ELISA protein-chip were 76.32%,97.20% and 93.64%,respectively.The positive and negative predictive rate was 95.08% and 85.25%.The positive rates of pulmonary tuberculosis patients were higher than those of the interference group and normal individuals(P﹤0.05).The sensitivity,precision and negative predictive rate of combined detection were significantly higher than each of these antigens alone(all P﹤0.05).However,the specificity and the positive predictive rate of combined detection was not of prognostic significance between different groups(all P﹥0.05).CONCLUSION Array-ELISA technology is a simple,rapid and sensitive method for detection of TB,and it might be an ideal method for assistant diagnosis of pulmonary tuberculosis in clinic.