游离脂肪酸对人近曲肾小管上皮细胞HK-2的影响及作用机制

目的探讨游离脂肪酸（Free Fatty Acids，FFAs）对人近曲肾小管上皮细胞（HK-2）的影响及作用机制。方法用含有不同浓度（0、125、250、500μmol／L）软脂酸的DMEM—F12培养人近曲肾小管上皮细胞（HK-2）在0h、24h、48h三个时段：MTT法检测不同环境下FFAs对HK-2细胞增殖的影响，流式细胞术检测FFAs对细胞凋亡的影响，免疫细胞化学法检测处理前后HK-2细胞中诱导型一氧化氮合酶（iNOS）及凋亡相关蛋白caspase8蛋白表达的变化。结果FFAs呈剂量和时间依赖性抑制HK-2细胞的增殖（P〈0．05），并可诱导细胞凋亡（P〈0．05）。经FFAs作用后，iNOS及Caspase8的表达增加。结论游离脂肪酸有抑制HK-2细胞增殖，促进其凋亡的作用，FFAs可能通过激活肾小管上皮细胞内的诱导型一氧化氮合酶（iNOS），加剧肾小管上皮细胞的凋亡，而caspase8参与了其发生和发展的过程。

Influence and Mechanism of Free Fatty Acid on Human Renal Proximal Tubular Epithelial Cells HK-2

Objective To explore the influence and function mechanism of free fatty acid （FFAs） to proximal convoluted tubules ep- ithelial cells（HK-2）. Methods People proximal convoluted tubules epithelial ceils（HK-2）,were cultured with DMEM-F12 of different concentrations of palmitic acid （0,125,250,500 μmol/L）（using defat bovine serum albumin as vector）, normal medium as control group on the 0h.24h.48h respectively：applyied four methyl azo thiazole blue（determined by MTT） colorimetric detection different environment HK-2 cell proliferation capacity;Flow cytometric intraoperativewas used to observe apoptosis influence.Immune cell chemical method before and after treatment were detected HK-2 ceils iNOS and caspase8 protein expression changes. Results A FFAs doses and time dependence inhibited promoting HK-2 cell proliferation （P〈0.05）,its apoptosis rate rised,compared with control signiilcantly increased （P〈0.05）.When the FFAs was working, and apoptosis related proteins iNOS Caspase8 expression increased. Conclusion Free fatty acid had inhibitory HK-2 cell proliferation and promoted its apoptosis rule,FFAs may through activating renal tubular epithelial cells within the iNOS,increased renal tubular epithelial cell apoptosis,but caspase8 participated in its occurrence and development process.