中医不同治法对四氧嘧啶糖尿病模型大鼠的降糖作用

目的：观察以滋阴清热为主的玉女煎、以养阴为主的左归丸和以滋阴温阳为主的肾气丸对实验性糖尿病大鼠模型的降糖作用,比较滋阴清热、养阴、滋阴温阳3种不同治法对于四氧嘧啶糖尿病模型大鼠的治疗作用。方法：采用四氧嘧啶制作糖尿病大鼠模型,造模成功的大鼠随机分为模型组和3组中药复方治疗组,治疗组动物分别给予玉女煎、左归丸和肾气丸三方水煎液灌胃治疗10d,葡萄糖氧化酶法测定动物空腹血糖,并行葡萄糖耐量实验检测各组实验动物对葡萄糖刺激的反应。结果：四氧嘧啶模型大鼠血糖水平较正常对照组明显升高（P〈0.05）;玉女煎、左归丸和肾气丸治疗后糖尿病大鼠血糖水平较模型组动物明显降低（P〈0.05）,玉女煎治疗组大鼠血糖水平较治疗前明显降低（P〈0.05）;葡萄糖耐量实验显示模型组及3组中药复方治疗组大鼠对葡萄糖刺激的反应较正常大鼠明显减弱,模型组与用药组之间差异无统计学意义。结论：玉女煎、左归丸和肾气丸对于四氧嘧啶导致的糖尿病大鼠均表现出明显的降糖效果,玉女煎降糖效果最佳。葡萄糖耐量实验显示降糖复方对于四氧嘧啶引起的糖代谢异常无改善作用。

Comparative study on hypoglycemic effects of different traditional Chinese medicine treatments in rats with diabetes mellitus induced by alloxan

Objective：To observe hypoglycemic effects of Yunu Decoction,Zuogui Pill and Shenqi Pill,three compound traditional Chinese herbal medicines,in treatment of diabetes mellitus induced by alloxan in rats,and to compare the therapeutic effects among the three recipes for nourishing yin,clearing away heat,and nourishing yin and warming yang.Methods：Diabetes mellitus was induced in rats with alloxan at a dose of 60 mg/kg via tail vain injection.The diabetic rats were randomly divided into four groups：alloxan model group,Yunu Decoction-treated group,Zuogui Pill-treated group and Shenqi Pill-treated group.Rats in the three recipe groups were administered intragastrically with water extraction of Yunu Decoction,Zuogui Pill,and Shenqi Pill accordingly for 10 days.Then the level of blood glucose was measured by glucose oxidase method and the glucose tolerance was determined.Results：Compared with the normal rats,blood glucose level in the alloxan model group was obviously increased （P0.05）.Glucose levels in the three recipe groups were obviously decreased as compared with the alloxan model group （P0.05）,and glucose level in the Yunu Decoction-treated group after treatment was significant lower than before treatment （P0.05）.The glucose tolerance test indicated that rats in the alloxan model and three recipe groups revealed impaired glucose tolerance as compared with the normal rats,and there were no significant differences between the alloxan model group and the three recipe groups.Conclusion：Yunu Decoction,Zuogui Pill and Shenqi Pill can effectively decrease the glucose level of the rats with diabetes mellitus induced by alloxan,and Yunu Decoction showed the best therapeutic effects.The glucose tolerance test shows that the three recipes cannot correct the abnormal metabolism of glucose.