弹性蛋白酶损伤肺致炎的地鼠血清中一氧化氮和肿瘤坏死 …

目的 观察由弹性蛋白酶损伤肺致炎的地鼠血清中一氧化氮（ＮＯ）和肿瘤坏死因子（ＴＮＦ－α）的动态变化,并初步探讨两者在炎症中的作用。方法 建立弹性蛋白酶损伤肺致炎金黄地鼠模型,用组化和免疫组化方法检测气道上皮细胞和肺内单核巨噬细胞一氧化氮合酶活性,测定血清中ＮＯ和ＴＮＦ－α的动态变化。结果 弹性蛋白酶损伤肺致炎地鼠气道上皮细胞和肺内单核巨噬细胞ＮＯＳ活性明显加强,在炎症早期血清中ＮＯ和ＴＮＦ－α含量

Changes of plasma nitric oxide and tumor necrosis factor-alpha in lung elastase injury induced inflammatory response of hamster

OBJECTIVE: To investigate the role of the NO and TNF-alpha in lung elastase injury induced inflammatory response of hamster. METHODS: Lung elastase injury induced inflammatory hamster model was established. NOS activity in airway epithelial cells and lung monocytes/macrophage, as well as the serum content of NO and TNF-alpha were measured respectively. RESULTS: The results indicated that iNOS activity in epithelium and monocytes/macrophages were significantly enhanced. In the early period of inflammation the NO and TNF-alpha content in serum increased obviously and maintained in a high level (P < 0.001). CONCLUSIONS: This Study suggested that both NO and TNF-alpha are important mediators participating in the lung elastase injury-induced inflammatory response. Our data suggested NO might play a regulatory role in the release of TNF-alpha.