| HPLC法测定肾石通颗粒中槲皮素的含量 |
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| 引用本文： | 罗爱勤,陈泽锋. HPLC法测定肾石通颗粒中槲皮素的含量[J]. 中国执业药师, 2012, 9(3): 17-19 |
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| 作者姓名： | 罗爱勤 陈泽锋 |
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| 作者单位： | 广州汉方现代中药研究开发有限公司,广东,广州,510240 |
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| 基金项目： | 广东省科技计划项目--以动态逆流提取和纳滤浓缩为核心的高效节能成套技术及装备的开发（2009A030901008） |
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| 摘 要： | 目的:建立高效液相色谱法测定肾石通颗粒中槲皮素含量的方法.方法:采用十八烷基硅烷键合硅胶色谱柱 (200 mm×4.6 mm),柱温30℃,流动相为甲醇-水(50∶50),流速1.0 mL/min,检测波长360 nm.结果:槲皮素在0.090 4～0.904 0 μg范围内质量与峰面积积分值呈良好线性关系,平均回收率98.8%,RSD = 1.71%.结论:该法灵敏、准确,可作为肾石通颗粒的质量控制方法.
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| 关 键 词： | 高效液相色谱法 肾石通颗粒 槲皮素 含量测定 |
Content Determination of Quercetin in Shenshitong Granules by HPLC |
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| Luo Aiqin , Chen Zefeng. Content Determination of Quercetin in Shenshitong Granules by HPLC[J]. China Licensed Pharmasist, 2012, 9(3): 17-19 |
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| Authors: | Luo Aiqin Chen Zefeng |
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| Affiliation: | (Guangzhou Hanfang Pharmaceutical Co, Ltd., Guangdong Guangzhou 510240, China ) |
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| Abstract: | To develop a method for the content determination of quercetin in Shenshitong Granules by HPLC. Methods： Chromatographic column （200 ram~4.6 mm） of octadecylsilane chemically bonded silica was used. The column temperature was at 30 ~C, the mobile phase was methanol-water （50： 50）,the flow rate was 1.0 mL/min,and the detection wavelength was at 360 nm. Results： There was a good linear relationship between the quality and peak area integral value in the range of 0.090 4~0.904 0 txg for quercetin. The average recovery rate was 98.8% （RSD = 1.71%）. Conclusion： The method is sensitive, accurate and can be used for quality control of Shenshitong Granules. |
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| Keywords: | HPLC Shenshitong Granules Quercetin Content Determination |
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