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| 荧光分光光度法快速测定食品和饲料蛋白质中的色氨酸 | |
| 引用本文： | 刘玉群,胡坚. 荧光分光光度法快速测定食品和饲料蛋白质中的色氨酸[J]. 营养学报, 1986, 0(2) |
| 作者姓名： | 刘玉群 胡坚 |
| 作者单位： | 上海市食品研究所 上海(刘玉群)，上海市食品研究所 上海(胡坚) |
| 摘 要： | 本文报告了食品和饲料中色氨酸的直接快速测定法。将1～5mg干样置于聚四氟乙烯管中,用含0.5％淀粉的5N NaOH溶液在145℃,真空水解4小时。水解产物用6N HCl在冰浴内中和,然后用4M尿素溶液(pH11)稀释5倍。在激发波长280nm,测368nm处的荧光强度。方法灵敏度为5ng/10ml。在1～5μg/10ml标准色氨酸范围内,回收率为97～107％。用此法测得食品和饲料中色氨酸值与文献相符。 |
| 关 键 词： | 色氨酸 食品 荧光分光光度法 |
A RAPID METHOD FOR DETERMINATION OF TRYPTOPHAN IN FOODS AND FEEDSTUFFS WITH FLUORIMETRIC METHOD |
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| Liu Yuequn,Hu Jian. A RAPID METHOD FOR DETERMINATION OF TRYPTOPHAN IN FOODS AND FEEDSTUFFS WITH FLUORIMETRIC METHOD[J]. Acta Nutrimenta Sinica, 1986, 0(2) | |
| Authors: | Liu Yuequn Hu Jian |
| Abstract: | A rapid method for directly determining tryptophan contents in foods and feedstuffs was described. l-5mg dry sample could be completely hydro-lyzed in a teflon tube containing 5N NaOH with 0.5% soluble starch under 145℃ for 4 hours in vacuum (760mmHg). The hydrolysate was then adjusted to neutral with 6N HCL in icebath and diluted by five times with 4M urea solution (pH = 11), and was measured at excitation wavelength 280nm and fluorescence wavelength 368nm. The sensitivity of the method was 5ng /10ml, and the recovery rate of 97-107% were obtained in the range of 1-5 μg/10ml standard tryptophan. The value of a number of foods and feed-stuffs obtained were in agreement with the literatature values. |
| Keywords: | tryptophan food fluorimetry |
| 本文献已被 CNKI 等数据库收录！ | |