HPLC法同时测定不同产地五味子生品及醋炙品中5个主要活性成分的含量

目的:建立HPLC法同时测定不同产地五味子生品及醋炙品中五味子醇甲、五味子醇乙、五味子甲素、五味子乙素和五味子丙素5个主要活性成分的含量。方法:采用Diamond C18色谱柱(250 mm×4.6 mm,5μm),以乙腈(A)-水(B)为流动相,梯度洗脱,流速:1.0 ml·min-1,检测波长:217 nm,柱温:30℃。结果:5种被测木脂素成分分离度良好;各成分质量与峰面积在测定范围内均呈良好的线性关系(r≥0.9994),重复性良好;平均回收率分别为98.2%,99.1%,101.8%,99.2%,101.2%,RSD分别为1.17%,0.80%,1.65%,1.49%,0.87%(n=6)。结论:该方法分离效率高,重复性好,经方法学验证,可用于五味子药材的质量控制。

Simultaneous Determination of Five Main Active Constituents in Schisandrae Chinensis Fructus from Different Habitats before and after Vinegar Processing

Objective:To establish an HPLC method to simultaneously determine schisandrin,schisandrol B,deoxyschizandrin,schisandrin B and schisandrin C in Schisandrae Chinensis fructus from different habitats before and after vinegar processing.Methods:A Diamond C18(250 mm×4.6 mm,5μm)column was used.The mobile phase consisted of acetonitrile-water in a gradient elution mode at the flow rate of 1.0 ml·min-1.The detection wavelength was at 217 nm.The column temperature was at 30℃.Results:Excellent chromatographic separation was achieved for five studied lignans with good linearity(r≥0.9994)within the studied concentration ranges.The average recovery was 98.2%,99.1%,101.8%,99.2%and 101.2%,and the RSDs were 1.17%,0.80%,1.65%,1.49%and 0.87%,respectively(n=6).Conclusion:Based on the method validation,the method with high separation efficiency and good repeatability can be used for the quality control of Schisandrae Chinensis fructus.