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| 他克莫司对截肢创伤后大鼠肝、肾组织线粒体功能的保护及其机制 | |
| 引用本文： | 张颖,张海峰,任青爱,刘宁,孙愚,谢晓华. 他克莫司对截肢创伤后大鼠肝、肾组织线粒体功能的保护及其机制[J]. 解放军保健医学杂志, 2011, 0(3) |
| 作者姓名： | 张颖 张海峰 任青爱 刘宁 孙愚 谢晓华 |
| 作者单位： | 解放军总医院南楼临床部综合外一科; |
| 基金项目： | 军队“十一五”科技攻关课题(06G117) |
| 摘 要： | 目的研究截肢创伤后大鼠肝、肾等远隔器官组织中线粒体的损伤情况,探讨他克莫司(FK506)的应用对受损线粒体的作用。方法将雄性SD大鼠随机分为正常对照组、截肢后6h组、FK506干预组,每组8只。通过手术制备大鼠左后肢截肢模型。大鼠处死后迅速提取肝、肾组织线粒体,测定呼吸控制率、膜电位及三磷酸腺苷(ATP)酶活性,留取截肢后6h组肝、肾组织,电镜观察线粒体的结构。结果电镜下可见截肢后6h组大鼠肝、肾实质细胞形态、结构基本正常,但细胞内线粒体出现一定程度的损伤。与正常对照组比较,截肢后6h组肝、肾组织细胞呼吸控制率(6.09±1.18、4.77±0.65vs2.76±0.82、3.07±0.94)、膜电位(247.83±12.68、246.65±15.21vs203.23±21.51、204.54±13.40)及ATP酶活性(14.01±2.41、11.60±2.42vs8.36±1.92、6.77±1.26)显著降低(P<0.01),FK506干预组肝、肾以上指标(呼吸控制率:3.74±0.71、4.54±0.73;膜电位:243.84±7.07、245.40±6.63,ATP酶活性:10.04±1.77、10... |
| 关 键 词： | 他克莫司 截肢 线粒体 损伤 |
Protection and mechanism of tacrolimus to the injury of mitochondria in liver and kidney of rats suffered amputation |
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| ZHANG Ying,ZHANG Hai-feng,REN Qin-ai,et al. Protection and mechanism of tacrolimus to the injury of mitochondria in liver and kidney of rats suffered amputation[J]. Journal Of Health Care And Medicine in Chinese Pla, 2011, 0(3) | |
| Authors: | ZHANG Ying ZHANG Hai-feng REN Qin-ai et al |
| Affiliation: | ZHANG Ying,ZHANG Hai-feng,REN Qin-ai,et al (First Department of Surgery,South building,Chinese PLA General Hospital,Beijing 100853,China) |
| Abstract: | Objective To investigate the hepatic and renal mitochondrial injury of rats suffering from amputation and the protective effect of tacrolimus(FK506)on chondriosome. Methods Twenty four male SD rats were divided into 3 groups at random(n=8):control group,6-hour-after-amputation group,and FK506 intervening group(0.1 mg/kg). The rats were sacrificed 6 hours after trauma,and the mitochondria were extracted from livers and kidneys. The mitochondrial respiratory control ratio(RCR),membrane potentials and total AT... |
| Keywords: | Tacrolimus Amputation Mitochondria Injury |
| 本文献已被 CNKI 等数据库收录！ | |