精子顶体酶检测在男性不育症辅助诊断中的应用价值

目的分析精子顶体酶检测在男性不育症辅助诊断中的应用价值和临床意义。方法选择2017年1月至2017年12月于我院就诊的218例男性不育症患者作为观察组,根据精液常规检查结果将其分为精液参数正常不育组(n=80)和精液参数异常不育组(n=138),并选取同一时段于我院其他科室就诊的114例可生育男性作为对照组。对所有入组研究对象的精子顶体酶结果进行统计分析,比较不同组顶体酶检测结果。结果精液参数正常不育组、精液参数异常不育组及对照组的精液顶体酶水平分别为(30.51±17.32)、(17.02±6.35)、(36.68±20.84)U/L,组间比较,差异具有统计学意义(P<0.05);对照组的精子顶体酶阳性率及酶活性亮区直径均优于精液参数正常不育组和精液参数异常不育组,差异具有统计学意义(P<0.05)。结论精子顶体酶活性低下是导致男性不育症的主要原因;精子顶体酶检测不仅能评估精子的受精能力,而且可为男性不育症的辅助诊断提供参考依据,值得临床推广应用。

Application value of sperm acrosin detection in auxiliary diagnosis of male infertility

Objective To analyze the application value and clinical significance of sperm acrosin detection in auxiliary diagnosis of male infertility. Methods A total of 218 male infertility patients treated in our hospital from January 2017 to December 2017 were selected as observation group. According to the results of routine semen examination, the patients were divided into infertility with normal semen parameters group(n =80) and infertility with abnormal semen parameters group(n =138). And 114 male patients with normal reproductive function in other departments of our hospital at the same time were selected as control group. Statistical analysis was made on the results of acrosomal enzymes in sperm of all study objects, and the examination results of acrosin among the different groups were compared. Results The levels of acrosin in the infertility with normal semen parameters group, the infertility with abnormal semen parameters group and the control group were(30.51 ±17.32),(17.02 ±6.35) and(36.68 ±20.84) U/L respectively, and there was significant difference among the three groups( P <0.05). The positive rate and the diameter of enzymatic activity bright areas of sperm acrosin in the control group were better than those of the infertility with normal semen parameters group and the infertility with abnormal semen parameters group, and the differences were statistically significant(P <0.05). Conclusion Low sperm acrosin activity is the main cause of male infertility. Sperm acrosin detection can not only evaluate the fertilization ability of sperm, but also provides reference for the auxiliary diagnosis of male infertility, which is worthy of clinical promotion and application.