| Iressa抑制胃癌SGC-7901细胞生长的研究 |
| |
| 引用本文： | 姚煜,田涛,李春丽,锁爱莉,南克俊. Iressa抑制胃癌SGC-7901细胞生长的研究[J]. 陕西医学杂志, 2008, 37(4): 459-461 |
| |
| 作者姓名： | 姚煜 田涛 李春丽 锁爱莉 南克俊 |
| |
| 作者单位： | 西安交通大学医学院第一附属医院肿瘤内科,西安,710061 |
| |
| 摘 要： | 目的:探讨iressa对胃癌SGC.7901细胞增殖,细胞周期和细胞凋亡的影响。方法:用四氮甲唑蓝(MTT)法检测不同浓度iressa对胃癌SGC-7901细胞增殖的影响;用流式细胞仪检测不同浓度iressa对胃癌SGC-7901细胞周期分布和细胞凋亡的影响。结果:在5~20μmol/L浓度范围内,iressa对SGC-7901的生长具有明显的抑制作用,并呈时间和剂量依赖性。10μmol/L和20μol/L的iressa可以导致SGC-7901细胞Gl期阻滞,并引起细胞凋亡。结论:Iressa可改变SGC-7901细胞周期分布,诱导细胞凋亡,从而抑制细胞增殖。
|
| 关 键 词： | 胃肿瘤/免疫学 苯胺化合物/药理学 细胞凋亡/免疫学 细胞周期 细胞分裂 @SGC-7901细胞 受体,表皮生长因子 |
| 修稿时间： | 2007-08-16 |
Experimental study on iressa inhibits the proliferation of gastric cancer SGC-7901 cells |
| |
| Affiliation: | Xi'an 710061 |
| |
| Abstract: | Objectives:To investigate the effect of iressa on the proliferation,cell cycle and apoptosis of human gastric cancer SGC-7901 cells.Methods:The proliferation inhibition of SGC-790 1 cells exposed to different concentration of iressa was observed by MTT assay.The cell cycle and apoptosis of SGC-7901 cells exposed to different concentration of iressa was analyzed by flowcytometry.Results:Within the rang of 5-20 umol/L,iressa obviously inhibited the proliferation of SGC--790 1 cells in a time--and dose--dependent manner.10umol/L and20 umol/L iressa caused SGC-790 1 cells to undergo G1 arrest and induced apoptosis.Conclusion:Iressa could inhibited SGC-7901 cells proliferation,caused G1 arrest and induced apoptosis. |
| |
| Keywords: | Stomach neoplasms/immunology Aniline compounds/pharmacology Apoptosis/immunology Cell cycle Cell division @SGC-7901ceu Receptor epidermol growth factorr |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
