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Bovine lactoferrin allergenicity as studied in murine model of allergy
Authors:Hanane Negaoui  Kamel Eddine El Mecherfi  Sid Ahmed Tadjer  Hadria Grar  Omar Kheroua
Affiliation:Laboratory of the Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Natural and Life Science, University of Oran 1, Ahmed Ben Bella, Oran, Algeria
Abstract:
In our study, we tried to investigate the allergenic properties of bovine lactoferrin (bLf) at different doses. The lactoferrin (LF) allergenicity was explored using murine model of allergy through measuring anti-LF IgG, IgG1 and IgE antibodies (Abs) responses and by in vivo anaphylactic reactions in LF sensitized mice. A histological examination of intestinal lamina propria was performed to assess gut inflammation. Four groups of mice (Balb/c) were sensitized intraperitoneally to bLf using different doses (1%, 2%, 5% and 10%). Histological study of intestinal lamina propria revealed a marked decrease in villus length in 5% and 10% sensitized mice groups. Also, high levels of anti-bLf IgG and IgE were observed. Concerning the in vivo reactions, all groups developed clinical symptoms of anaphylactic reactions at different stages. Our findings show that LF-sensitized mice at 5% and 10% developed important clinical symptoms after intraperitoneal challenge with LF.
Keywords:Lactoferrin  allergen  Balb/c  gut inflammation  anaphylactic reaction
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