支气管哮喘患者中性粒细胞与白细胞介素8,6动态监测及其意义

目的 :探讨中性粒细胞 (PMN)与白细胞介素 8(IL - 8)、白细胞介素 6 (IL - 6 )在支气管哮喘 (哮喘 )气道炎症反应的作用以及与哮喘的关系。方法 :对哮喘患者急性发作期和缓解期血清、肺泡灌洗液 (BALF)的PMN及IL - 8,IL - 6浓度测定 ,分别与健康志愿者进行对比。结果 :哮喘急发期组血清IL - 8水平和IL - 6水平明显比哮喘缓解期组及健康志愿组高 (P <0 .0 1) ,哮喘缓解期组血清IL - 8水平比健康志愿组高 (P <0 .0 5 )。哮喘缓解期组血清IL - 6水平与健康志愿组比较差异无显著性 (P >0 .0 5 )。BALF的PMN ,IL - 8和IL - 6水平哮喘急发期组比哮喘缓解期组及健康志愿组高 (P <0 .0 1) ,哮喘缓解期组比健康志愿组高 (P <0 .0 5 )。哮喘患者BALF的IL - 8水平与PMN呈显著正相关 (r=0 .80 36 ) ,BALF的IL - 6水平与IL - 8水平呈显著正相关 (r=0 .76 15 )。BALF的IL - 8水平、IL - 6水平、PMN分别与FEV1占预计值 %呈显著负相关(r=- 0 .785 2 ,r=- 0 .6 5 34,r=- 0 .8891)。结论 :PMN ,IL - 6及IL - 8参与哮喘的气道炎症反应 ,而且哮喘缓解期患者的气道炎症反应持续存在。

DYNAMIC DETECTION AND SIGNIFICANCE OF NEUTROPHILS,INTERLEUKIN-8,6 FROM PATIENTS WITH ASTHMA

Objective:To evaluate the role and relationship of neutrophils(PMN) and interleukin-8 (IL-8), interleukin-6 (IL-6) in pathogenesis of asthma.Methods:Bronchoalveolar lavage fluid (BALF) and plasma were obtained from 28 patients with unstable asthmatics and stable asthmatics, 12 normal people. PMN of BALF were examined .The levels of IL-8 andIL-6 in plasma and BALF were measured by enzyme-linked immunosorbent assay (ELISA).Results:The levels of plasma IL-8 and IL-6 of unstable asthmatics were significantly higher than those of stable asthmatics and normal subjects ( P <0.01). Compared with the normal subjects, there was a significant increase in the level of plasma IL-8 from stable asthmatics ( P <0.05),but the level of plasma IL-6 did not differ significantly from that of normal subjects ( P >0.05). PMN, levels of IL-8 and IL-6 in BALF from unstable asthmatics were significantly higher than those of stable asthmatics and normal subjects ( P <0.01), and those from stable asthmatics were significantly higher those from normal subjects ( P <0.05). The level of IL-8 in BALF was correlated positively with PMN (r = 0.8036).The level of IL-6 in BALF showed a positive correlation with the level of IL-8 in asthmatics (r = 0.7615). The levels of IL-8 and IL-6, PMN in BALF were all correlated negatively with FEV~% in asthmatics (r = -0.7852, -0.6534, -0.8417).Conclusions:PMN, IL-8 and IL-6 participate in development of airway inflammation in asthma. The airway inflammation still remains in stable asthmatics.