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| 人牙周膜成纤维细胞原代培养的研究 | |
| 引用本文： | 胡军,陈新民,麻健丰. 人牙周膜成纤维细胞原代培养的研究[J]. 现代口腔医学杂志, 2002, 16(2): 176-177 |
| 作者姓名： | 胡军 陈新民 麻健丰 |
| 作者单位： | 1. 310006,浙江大学附属口腔医院修复科 2. 华西医科大学附属口腔医院 3. 温州医学院附属第一医院 |
| 摘 要： | 目的：研究如何提高人中膜成纤维细胞原代培养的成功率。方法：以牙槽窝刮取组织块法替代传统的牙根刮取法，以组织块自然贴壁法替代传统的干燥贴壁法。结果：48h后细胞游出率为40％，而成功传代率为20％，结论：通过对取材和培养方法的改良，可以显著提高人牙周膜成纤维细胞原代培养的成功率。 |
| 关 键 词： | 原代培养 牙周膜成纤维细胞 组织块培养法 研究 |
| 修稿时间： | 2001-03-20 |
Primary culture of human periodontal ligament fibroblast |
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| HU Jun,CHEN Xinmin,MA Jiangfeng. Hospital of Stomatology,Zhejiang University,Hangzhou. Primary culture of human periodontal ligament fibroblast[J]. Journal of Modern Stomatology, 2002, 16(2): 176-177 | |
| Authors: | HU Jun CHEN Xinmin MA Jiangfeng. Hospital of Stomatology Zhejiang University Hangzhou |
| Affiliation: | HU Jun,CHEN Xinmin,MA Jiangfeng. Hospital of Stomatology,Zhejiang University,Hangzhou 310006 |
| Abstract: | Objective To increase the success rate of primary culture of human periodontal ligament fibroblast.Methods The tissue was scraped from the alveolar socket in place of the surface of tooth root traditionally,the tissue was anchored to Petri dish naturally in place of the dry anchoring traditionally.Results The cell emigration rate of tissue was up to 40% after 48h and success rate to 20%.Conclusion By making modifications in culture methods and specimen collecting,we can noticeably increase the success rate of primary culture of human periodontal ligament fibroblast. |
| Keywords: | Primary culture Periodontal ligament fibroblast Tissue culture |
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