石榴皮中没食子酸定性定量方法研究

目的:建立石榴皮中没食子酸定性定量的方法。方法:用薄层色谱法对石榴皮有效成分没食子酸进行定性分析,采用高效液相色谱法测定了没食子酸含量。以DiamonsilC₁₈柱为固定相,流动相0.2%甲醇乙腈0.1%磷酸和0.1%三乙胺水溶液(3∶97),检测波长216nm,柱温40℃,结果:没食子酸在0.085～0.768μg峰面积与进样量呈良好的线性,r=0.9996;平均回收率96.7%;RSD0.8%。结论:通过20批不同产地石榴皮的鉴别和含量测定,证明方法灵敏准确可靠,重复性好。

Study on the qualitative and quantitative methods of gallic acid in pomegranate rind

OBJECTIVE: To establish the qualitative and quantitative methods of gallic acid in pomegranate rind. METHOD: The thin layer chromatographic method was used for identitication, and the high performance liquid chromatographic method was used for assay. Extracts were separated on a Diamonsil C18 column eluted with the mobile phase of a mixture of acetonitrile containing 0.2% methanol and water containing 0.1% phosphoric acid and 0.1% TEA (3:97). The detection wavelength was set at 216 nm, and colum temperature was 40 degrees C. RESULT: The calibration curve was linear in the range of 0.085-0.768 microg (r = 0.9996). The average recovery was 96.7% (RSD 0.8%, n = 5). 20 batches of the crude drug were identified and assayed, with the methods. CONCLUSION: The methods were sensitive and reliable, and can be used for quality control of the pomegranate rind.