Comparison of traditional and molecular methods for typing nontoxigenic strains ofCorynebacterium diphtheriae |
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| Authors: | P Riegel F I S Freitas G Prévost C Andronescu F Bimet M Kiredjian E Estrangin J P Emond S Dellion B Halioua H Monteil O Patey |
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| Institution: | (1) Institut de Bactériologie de la Faculté de Médecine, 3 rue Koeberlé, F-67000 Strasbourg, France;(2) Institut Cantacuzen, Bucarest, Romania;(3) Laboratoire des Identifications Bactériennes, Institut Pasteur, Paris, France;(4) Laboratoire de Microbiologie, C.H.I. Créteil, France;(5) Laboratoire de Microbiologie, C.H.I. Villeneuve-St-Georges, France;(6) Service des Maladies Infectieuses et Tropicales, C.H.I. Villeneuve-St-Georges, France |
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| Abstract: | Thirty-eight nontoxigenic strains ofCorynebacterium diphtheriae isolated between 1987 and 1992 from clinical specimens of French patients were typed by biotyping, antibiograms, bacteriophage
typing, ribotyping, and restriction analysis by pulsed-field gel electrophoresis (PFGE). Excellent correlation occurred between
the genotypes defined by PFGESfil profiles or by ribotypeBstEll profiles. Genotyping revealed seven genotype patterns among the 26 biotype mitis isolates, five among the nine biotype
gravis isolates, and three among the three biotype belfanti isolates. Phage typing was nonreactive for nine of the 38 isolates.
A combination of all the typing methods led to the identification of 19 different types ofCorynebacterium diphtheriae. |
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