扶正法、祛邪法对Lewis肺癌小鼠的抑瘤作用及机制研究

目的:研究扶正法、祛邪法对Lewis肺癌小鼠的抑瘤作用并探讨其作用机制。方法:建立Lewis肺癌小鼠移植瘤模型,按实验分组干预,计算各组抑瘤率,免疫组化法检测BCL-2表达情况,RT-PCR法检测Caspase-3 m RNA、Survivin m RNA表达情况。结果:芪贞固本方、芩莲正积方均有不同程度的抑瘤作用,其抑瘤率分别为18.42%、29.24%,两组比较差异无统计学意义(P0.05);二方均可促进Caspase-3 m RNA的表达,抑制BCL-2、Survivin m RNA的表达,与对照组比较有显著统计学意义(P0.05),但均不及DDP组。结论:芪贞固本方和芩莲正积方确有抗肿瘤作用,芩莲正积方作用略强,其机制可能为促进Caspase-3m RNA的表达,抑制BCL-2、Survivin m RNA的表达。

Study of the Anti-tumor Effect and Mechanism of Strengthening Healthy Qi and Eliminating Pathogenic Factors on Mice Model with Lewis Lung Cancer

Objective:To study of the anti-tumor effect and mechanism of strengthening healthy Qi and eliminating pathogenic factors on mice model with Lewis lung cancer. Method:The Lewis lung cancer mouse model was established,the mic were divided into different groups and given intervention. The anti-tumor rate of each group was calculated,the expression of BCL-2 in tumor cells was measured with immunohistochemistry,the expressions of caspase-3 m RNA,surviving m RNA were detected with real-time PCR. Results:Qizhen guben formula and Qinlian zhengji formula all had certain anti-tumor effect,with the anti-rate 18.42% and29.24% respectively,and there were no significant differences between two groups(P> 0.05). When compared to the control group,they all could significantly promote the expression of caspase m RNA and down-regulate the expression of BCL-2 survivin m RNA,but the their effect was not better than cisplatin. Conclusion:Qizhen guben formula and Qinlian zhengji formula did possess anti-tumor effect and this effect of Qinlian Zhengji formual was stronger. Their mechanism of action may be related to promoting the expression of caspase m RNA and down-regulating the expression of BCL-2 and survivin m RNA.