| 反相高效液相色谱法测定人血浆中丙基硫氧嘧啶浓度 |
| |
| 引用本文： | 黄英,魏松全.反相高效液相色谱法测定人血浆中丙基硫氧嘧啶浓度[J].华西药学杂志,1989,4(1):17-19. |
| |
| 作者姓名： | 黄英 魏松全 |
| |
| 作者单位： | 华西医科大学临床药理研究所 成都
(黄英，曾经泽)，华西医科大学附一院内科 成都(魏松全) |
| |
| 摘 要： | 本文报道用反相高效液相色谱法测定人血浆中丙基硫氧嘧啶浓度。用Ultrasphere-ODS柱,甲醇:水(30:70)为流动相,茶碱作内标,于254nm检测。萃取回收率为89.1%,方法回收率为98.1%,日内变异系数为5.9%,日间变异系数为7.2%。本法灵敏,准确,重现性好,已用于临床甲亢病人药代动力学研究的血药浓度测定,取得良好结果。
|
| 关 键 词： | 丙基硫氧嘧啶 血药浓度 液相色谱 |
DETERMINATION OF PROPYLTHIOURACIL IN HUMAN PLASMA BY REVERSE PHASED HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
| |
| Huang Ying,Zeng Jingze,Wei Songquan Clinical Pharmacology Institute,First Affiliated Hospital,west China University of Medical Sciences Chengdu.DETERMINATION OF PROPYLTHIOURACIL IN HUMAN PLASMA BY REVERSE PHASED HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J].West China Journal of Pharmaceutical Sciences,1989,4(1):17-19. |
| |
| Authors: | Huang Ying Zeng Jingze Wei Songquan Clinical Pharmacology Institute First Affiliated Hospital west China University of Medical Sciences Chengdu |
| |
| Abstract: | A reversed phase highperformance liquid chromatographic me-thod is described for measuring propylthi-ouracil level in human plasma. The pro-cedure involves a single extraction ofpropylthiouracil and internal standard(theophylline) from plasma. The eluentis monitored at 254nm. The mobile phaseconsists of methanol-water(30:70). Theextraction recovery is 89. 1%. Within dayCV and day to day CV are 5.8% and 7.2%respectively. The assay is sensitive,accu-rate and reliable. It has been used to de-termine the drug level in patient blood. |
| |
| Keywords: | Words propylthiouracil plasma concentration reversed phase HPLC |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
