褶合曲线分析法测定复方茶碱片中六种主要成分

应用柱分配色谱,将复方茶碱片分离出各含三个组分的两组混合物,然后分别应用褶合曲线分析法,同时定量测定了两组混合物。六个被测成分的平均百分回收率和相对标准差分别为:咖啡因101.6,1.46%;非那西丁99.7,0.10%;苯巴比妥100.9,1.31%;可可碱99.9,0.81%;茶碱100.2,0.81%;氨基比林100.8,0.48%。

DETERMINATION OF SIX MAIN COMPONENTS IN COMPOUND THEOPHYLLINE TABLET BY CONVOLUTION CURVE METHOD AFTER PRIOR SEPARATION BY COLUMN PARTITION CHROMATOGRAPHY

On a partition chromatographic column in which the support is Kieselguhr and the stationary phase is sulfuric acid solution (2 mol/L), three components of compound theophylline tablet were simultaneously eluted by chloroform and three other components were simultaneously eluted by ammonia--saturated chloroform. The two mixtures were determined by computer--aided convolution curve method separately.The corresponding average recovery and relative standard deviation of the six components were as follows: 101.6, 1.46% for caffeine; 99.7, 0. 10% for phenacetin; 100.9, 1.31% for phenobarbitone; 100. 2, 0. 81% for theophylline; 99.9, 0. 81% for theobromine and 100. 8, 0. 48% for aminopyrine.