小檗碱抑制谷氨酸引起的新生大鼠脑细胞 c-fos 表达及游离钙的升高

目的：研究小檗碱（Ｂｅｒ）对谷氨酸（Ｇｌｕ）引起的新生大鼠脑细胞ｃ－ｆｏｓ表达及游离钙（〔Ｃａ２＋〕ｉ）变化的影响。方法：斑点杂交及Ｆｕｒａ－２技术。结果：Ｇｌｕ能诱导分离的脑细胞ｃ－ｆｏｓ的一过性高表达及〔Ｃａ２＋〕ｉ的显著升高。Ｂｅｒ１０μｍｏｌ·Ｌ－１显著抑制Ｇｌｕ诱导的脑细胞ｃ－ｆｏｓ的高表达，而尼莫地平则没有明显作用。Ｂｅｒ１～１００μｍｏｌ·Ｌ－１能剂量依赖地抑制Ｇｌｕ引起的〔Ｃａ２＋〕ｉ升高。结论：Ｂｅｒ的这种降低脑细胞ｃ－ｆｏｓ基因表达及〔Ｃａ２＋〕ｉ水平的作用可能是其治疗脑缺血性疾病的机制之一。

Inhibitory effects of berberine on c fos expression and intracellular free calcium elevation induced by glutamate in dissociated brain cells of neonatal rats

AIM: Effects of berberine (Ber) on c fos mRNA expression and resting free intracellular Ca 2+ level induced by glutamate in isolated brain cells of neonatal rats were studied. METHODS: Dot blotting assay and Fura 2 fluorescence technique. RESULTS: Glu 10 μmol·L -1 significantly increased Ca 2+ i elevation and temporally induced c fos gene expression in isolated brain cells of neonatal rats. However, the dynamic changes of c fos gene expression could be inhibited dramatically by Ber 10 μmol·L -1 , but nimodipine did not have such effect. Ber 1￣100 μmol·L -1 significantly inhibited Glu induced Ca 2+ i elevation in dose dependent manner. CONCLUSION: The inhibitory actions of Ber on c fos expression and brain Ca 2+ i elevation induced by Glu may be related to its protective brain ischemia.