膀胱癌细胞在不同生长环境中癌干细胞相关标志物CD44、CXCR4表达情况的初步研究

目的 观察癌干细胞相关标志物CD44、CXCR4在不同环境下膀胱癌细胞的变化情况,为人体膀胱癌干细胞的进一步研究提供前期数据与背景资料.方法 采用免疫组织化学与流式细胞术等技术,对体外培养、皮下移植瘤以及裸鼠淋巴结转移模型等不同的生长微环境的膀胱癌5637细胞系癌干细胞相关标志物CD44、CXCR4的表达进行检测.结果 在体外培养环境中,膀胱癌5637的细胞膜蛋白CD44呈阳性表达,而CXCR4呈弱阳性表达;膀胱癌裸鼠移植瘤中CD44、CXCR4均呈阳性表达.与体外培养体系比较,流式细胞术检测发现膀胱癌细胞中5637的CD44阳性表达水平无显著变化,而CXCR4阳性表达水平显著高于体外培养体系（P＆lt;0.01）;在裸鼠淋巴结转移模型中,转移淋巴结均有CD44、CXCR4阳性表达.结论 膀胱癌细胞中部分癌干细胞相关标志物的变化受体内外不同环境变化的调控,也提示膀胱癌5637细胞系存在有癌干细胞.

Preliminary research on the expression of bladder cancer stem cell marker CD44 and CXCR4 in different environment

Objective To observe and collect the information of determination of bladder cancer stem cell marker CD44 and CXCR4. Methods Cell hne is bladder cancer 5637 cell hne. The different environments （niche） include cell culture system, the model of human bladder cancer xenograft, the metastatic lymph node model of blad- der cancer. CD44 and CXCR4 were detected by immunohistochemical staining and flow cytometry respectively. Re- suits In the bladder cancer 5537 cell line in vitro, CD44 was strong positive, and CXCR4 was low positive. In the model of xenograft, CD44 and CXCR4 were positive. Compared with the bladder cancer 5637 cell line in vitro, the positive expression of CD44 was no change and the positive expression of CXCR4 was higher （P 〈 0. 01 ）. In the metastatic lymph node model, CD44 and CXCR4 were positive. Conclusion The expression of bladder cancer stem cell markers are regulated by the different environments. Bladder cancer stem cells exist in the 5673 cell hne.