| UPLC-MS/MS检测大鼠体内达卢生坦的血药浓度及其药动学研究 |
| |
| 引用本文： | 冯炎林,田伟强,蒋晓梅. UPLC-MS/MS检测大鼠体内达卢生坦的血药浓度及其药动学研究[J]. 中国现代应用药学, 2018, 35(4): 506-509 |
| |
| 作者姓名： | 冯炎林 田伟强 蒋晓梅 |
| |
| 作者单位： | 温州医科大学附属五院丽水市中心医院药学部, 浙江 丽水 323000,温州医科大学附属五院丽水市中心医院药学部, 浙江 丽水 323000,温州医科大学附属五院丽水市中心医院药学部, 浙江 丽水 323000 |
| |
| 摘 要： | ![]()
目的 建立一种快速、高灵敏度和高选择性的UPLC-MS/MS用于大鼠血浆中达卢生坦的测定。方法 通过蛋白沉淀法完成血浆样品处理。通过Acquity UPLC BEH C18柱（2.1 mm×50 mm，1.7 μm）色谱柱，流动相为0.1%甲酸水-乙腈，流速为0.4 mL·min-1。采用ESI+方式检测，扫描方式为多反应离子监测方式检测。结果 达卢生坦血药浓度在0.01~2.5 μg·mL-1内线性关系良好（R2>0.999 6），定量下限（LLOQ）为0.01 μg·mL-1。血浆中的达卢生坦平均回收率为87.613%~97.115%，日内及日间精密度RSD<7.71%，基质效应RSD<9.34%。结论 该方法灵敏高，快速高效，能成功应用于大鼠口服10.0 mg·kg-1达卢生坦后的药动学研究。
|
| 关 键 词： | 达卢生坦 UPLC-MS/MS 大鼠血浆 药动学 |
| 收稿时间： | 2017-07-16 |
| 修稿时间： | 2018-03-01 |
Study on Plasma Concentration and Pharmacokinetics of Darusentan in Rat Plasma by UPLC-MS/MS |
| |
| Feng Yanlin,Tian Weiqiang and Jiang xiaomei. Study on Plasma Concentration and Pharmacokinetics of Darusentan in Rat Plasma by UPLC-MS/MS[J]. The Chinese Journal of Modern Applied Pharmacy, 2018, 35(4): 506-509 |
| |
| Authors: | Feng Yanlin Tian Weiqiang Jiang xiaomei |
| |
| Affiliation: | Department of phmarcy, the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui Municipal Central Hospital, Lishui 323000, china,Department of phmarcy, the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui Municipal Central Hospital, Lishui 323000, china and Department of phmarcy, the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui Municipal Central Hospital, Lishui 323000, china |
| |
| Abstract: | ![]()
|
| |
| Keywords: | darusentan UPLC-MS/MS rat plasma pharmacokinetic |
|
| 点击此处可从《中国现代应用药学》浏览原始摘要信息 |
|
点击此处可从《中国现代应用药学》下载全文 |
|
