电针治疗周围神经损伤后损伤局部差异表达基因分析

目的] 通过分析电针治疗周围神经损伤后损伤局部差异表达基因，进一步阐明电针治疗周围神经损伤机制。方法] 选取大鼠坐骨神经横断伤模型为研究对象，随机分为空白组、模型组、电针模型组，于电针6 d后取损伤处坐骨神经，通过高通量技术筛选差异基因，对差异基因进行GO功能分析、KEGG通路分析。结果] 模型组与空白组相比，上调差异基因3 001个，下调差异基因1 169个。电针模型组与模型组相比，表达上调差异基因24个，下调差异基因80个，结合GO分析结果和KEGG富集分析结果，筛选出了可能与电针治疗周围神经损伤相关的8个通路：白细胞跨内皮迁移通路、γ-氨基丁酸能突触通路、胆碱能突触通路、5-羟色胺突触通路、钙信号通路、紧密连接通路、黏着斑通路、肌动蛋白细胞骨架的调控通路。结论] 电针可能是通过改变细胞电位平衡、神经元轴突再生骨架结构的重建以及白细胞的迁移而改变周围神经损伤后修复与再生的微环境，从而促进了受损神经的再生。

Analysis of local differentially expressed genes in peripheral nerve injury treated by electroacupuncture

Objective] To elucidate the mechanism of electroacupuncture in the treatment of peripheral nerve injury by analyzing the locally differentially expressed genes after the treatment of peripheral nerve injury.Methods] A rat model of sciatic nerve transection injury was selected and randomly divided into a blank group, a model group and an electroacupuncture model group. The sciatic nerve at the site of injury was taken 6 days after electroacupuncture. The differentially expressed genes were screened by high-throughput technology, and the differentially expressed genes were analyzed by GO function analysis and KEGG pathway analysis.Results] Compared with the blank group, three thousand and one differentially expressed genes were up-regulated and 1 169 differentially expressed genes were down-regulated in the model group. Cupping model group compared with model group, expression level difference 24 genes, genetic variations cut 80, GO analysis results and KEGG enrichment analysis results, the screen may be associated with cupping in the treatment of peripheral nerve injury eight pathways:leukocyte transendothelial pathways, serotonergic synapse pathways, calcium signaling pathway, tight junction pathways, focal adhesion pathway and regulation of actin cytoskeleton pathway.Conclusion] Electroacupuncture may change the microenvironment of peripheral nerve repair and regeneration by changing the cell potential balance, the regeneration of axon regenerated skeleton structure and the migration of white blood cells, thus promoting the regeneration of damaged nerve.