干扰CD151基因表达对裸鼠宣威肺腺癌XWLC-05细胞肺转移的影响

目的 探讨RNA干扰抑制CD151基因表达对宣威肺腺癌XWLC-05细胞侵袭和迁移的影响及裸鼠肺转移的实验。方法 构建能高效抑制CD151基因表达的3组重组干扰质粒，筛选出稳定转染干扰质粒的宣威肺腺癌XWLC-05细胞株。MTT、划痕实验、Transwell实验研究干扰CD151基因表达前后对细胞迁移和侵袭的影响。将构建的RNA干扰抑制CD151基因表达的稳转细胞XWLC-05-shRNA、XWLC-05-shNC、XWLC-05-Blank通过尾静脉注射入BALB/c裸鼠，30天后通过肉眼和HE染色后观察肺转移情况。结果 划痕实验和Transwell实验表明RNA干扰抑制CD151基因表达可能抑制宣威肺腺癌XWLC-05细胞体外迁移和侵袭作用（P<0.05）。XWLC-05-shRNA、XWLC-05-shNC、XWLC-05-Blank各组裸鼠肺转移率及肺组织上的转移病灶数依次为75%（6/8）与（5.2±1.2）、100%（8/8）与（9.8±1.6）、87.5%（7/8）与（9.1±1.2）。XWLC-05-shNC与XWLC-05-Blank组比较差异无统计学意义（P=0.423），XWLC-05-shRNA与XWLC-05-shNC（P=0.000）和XWLC-05-Blank组比较差异有统计学意义（P=0.000）。结论 RNA干扰抑制CD151基因表达能抑制XWLC-05细胞的体外迁移和侵袭；能减少XWLC-05细胞裸鼠的肺转移。CD151可能成为抑制宣威肺癌侵袭和迁移的潜在治疗靶点之一。

Influence of CD151 Inhibition by RNAi on Lung Metastasis in Nude Mice Given Tail Veins Injection of Xuanwei Lung Adenocarcinoma XWLC-05 Cell Lines

Objective To explore the influence of CD151 inhibition by RNA interference on invasion and metastasis of Xuanwei lung adenocarcinoma XWLC-05 cells, as well as lung metastasis in nude mice given tail veins injection of XWLC-05 cells. Methods Recombinant plasmid aimed to interfere CD151 gene was constructed and transfected into XWLC-05 cells. MTT, wound-healing assay and Transwell assay were applied to detect cell proliferation and migration abilities. Stably-expressed XWLC-05-shRNA, XWLC-05-shNC and XWLC-05-Blank cell lines were injected into the tail veins of BALB/c nude mice. Lung metastasis was measured by naked eye and microscope after HE staining. Results Inhibition of CD151 gene expression could reduce the migration and invasion abilities of XWLC-05 cells in vitro(P<0.05). The proportion and number of lung metastasis were 75%(6/8) and (5.2±1.2), 100%(8/8) and (9.8±1.6), 75%(7/8) and (9.1±1.2) in XWLC-05-shRNA, XWLC-05-shNC and XWLC-05-Blank groups, respectively. There was no differences in XWLC-05-shNC vs. XWLC-05-Blank group(P=0.423). There were significant differences in XWLC-05-shRNA vs. XWLC-05-shNC group(P=0.000) and XWLC-05-shRNA vs. XWLC-05-Blank group(P=0.000). Conclusion Inhibition of CD151 gene expression could reduce lung metastasis in nude mice given tail veins injection of XWLC-05 cells. CD151 may become one of the potential therapeutic targets for inhibiting the invasion and metastasis of Xuanwei lung adenocarcinoma.