不同剂量的1，25（OH）2D3对大鼠Thy1肾炎系膜细胞凋亡的影响

目的：探讨不同剂量的1，25（OH）2D3对大鼠抗胸腺细胞抗体（Thy1）肾炎系膜细胞凋亡的影响。方法将SD大鼠120只分为空白对照组（A组）、肾炎模型组（B组）、低剂量1，25（OH）2D3组（C组）和高剂量1，25（OH）2D3组（D组），每组30只。C、D两组造模成功后给药干预，4组分别于干预后第1、3、7、14、21天每组随机处死6只大鼠，取肾组织标本经HE及PAS染色后确定肾脏病理损害分级；免疫组化法检测肾组织中半胱氨酸天冬氨酸蛋白酶3（Caspase‐3）的表达；TUNEL试剂盒检测其肾小球内细胞凋亡。结果与A组比较，B组Caspase‐3表达增强（P＜0．05）；与B组比较，C、D组Caspase‐3表达增强，但差异无统计学意义（P＞0．05）；D组表达最高，但与C组比较差异无统计学意义（P＞0．05）。与A组比较，B组肾小球内凋亡细胞明显增多（P＜0．05），且随时间推移逐渐增强；与B组比较，C、D两组凋亡明显增多（P＜0．05），并在3d达高峰，7、14d逐渐下降，21d趋于正常（P＜0．05）；C、D两组比较，D组凋亡细胞增多明显，但差异无统计学意义（P＞0．05）。结论1，25（OH）2D3具有诱导大鼠肾小球系膜细胞凋亡的作用，其参与了对Caspase‐3的调节，诱导系膜细胞凋亡，促进肾炎的修复，可延缓肾脏疾病进展。

Effects of different doses of 1,25 (OH)2 D3 on rat Thy-1 nephritis mesangial cell apoptosis

Objective To investigate the effects of different doses of 1 ,25(OH)2D3 on rat Thy‐1 nephritis mesangial cell ap‐optosis .Methods 120 clean SD rats were divided into the blank control group(group A) and the experimental group .The experi‐mental group was re‐divided into the nephritis group(group B) ,0 .25μg 1 ,25(OH)2D3 intervention group(group C) and 0 .50μg 1 , 25(OH)2D3 group(group D) ,30 cases in each group .The group C and D were performed the medication intervention after success‐fully constructing the model .6 rats were randomly killed in each group on 1 ,3 ,7 ,14 ,21 d after intervention .The renal tissues were taken for determining the renal pathological injury classification after hematoxylin and eosin staining and PAS staining .The expres‐sion of Caspase‐3 in the renal tissues was detected by the immunohistochemistry method ,and the glomerular cell apoptosis was de‐tected by TUNEL .Results The immunohistochemistry results showed that compared with the group A ,the Caspase‐3 expression in the group B was increased (P<0 .05);compared with the group B ,the Caspase‐3 expression in the group C and D was increased , but there was no statistical differences between them (P> 0 .05);the Caspase‐3 expression in the group D was highest ,but the difference was not statistically significant compared with the group C .The TUNEL results showed that compared with the group A ,the apoptotic glomerular cells in the group B were increase obviously (P<0 .05) ,which was gradually enhanced over time ;com‐pared with the group B ,the apoptotic glomerular cells in the group C and D were increased significantly (P<0 .05) ,reaching a peak on 3 d ,then decreasing gradually on 7 ,14 d and tending to be normal on 21 d(P<0 .05);the comparison between the group C and D showed that the apoptotic cells in the group D were increased significantly ,but the difference between these two group ,was not statistically significant(P>0 .05) .Conclusion 1 ,25(OH)2D3 has the effect for inducing glomerular mesangial cell apoptosis in rat , which participates in the regulation of Caspase‐3 ,induces glomerular mesangial cell apoptosis ,promotes the recovery of nephritis and could delay the progression of renal disease .