补充谷氨酰胺对过度训练大鼠腹膜巨噬细胞IGF-1和MGF基因表达的影响

目的:了解补充谷氨酰胺对过度训练大鼠腹膜巨噬细胞胰岛素样生长因子1(IGF-1)和机械生长因子(MGF)基因表达的影响。方法:8周龄健康雄性Wistar大鼠40只,随机分为安静对照组(C)、过度训练组(E)、过度训练补充谷氨酰胺组(EG)。后两组根据取材时间不同分为2组:运动后36 h取材组(E1、EG1),运动后7天取材组(E2、EG2)。总计5组,每组8只,除C组外,其他4组进行11周递增负荷跑台训练。EG组从第5周开始至第8周灌胃补充谷氨酰胺(0.8 g/kg/d),以后几周加至饮用水补充,剂量逐周加大到1.1 g/kg/d。断头处死大鼠并分离纯化腹膜巨噬细胞,采用荧光定量PCR技术测定IGF-1和MGF基因表达。结果:安静状态下巨噬细胞即可表达IGF-1和MGF。11周过度训练后36 h,巨噬细胞IGF-1、MGF表达量显著增加,分别约为安静对照组的21倍和92倍(P<0.01)。EG1组IGF-1、MGF表达显著增加,分别约为安静对照组的10倍和37倍(P<0.01),但显著低于E1组(P<0.01)。停训后恢复7天,E2组、EG2组IGF-1、MGF表达量分别与E1组、EG1组相比均显著下降(P<0.01),但与C组相比差异无统计学意义。结论:静息态巨噬细胞可表达IGF-1和MGF;过度训练可增强巨噬细胞IGF-1和MGF表达,MGF表达对运动应激更敏感;补充谷氨酰胺可部分抑制巨噬细胞IGF-1和MGF对过度训练的应答。

Supplementing Glutamine Affects the IGF-1 and MGF mRNA Levels in Peritoneal Macrophages of Overtrained Rats

Objective To investigate the effect of supplementation of glutamine(Gln)on the IGF-1 and MGF mRNA levels in peritoneal macrophages of rats with overtraining.Methods Forty male Wistar rats were randomly divided into following groups:sedentary group(C,n = 8),overtraining group(E),overtraining and supplementation of Gln group(EG).The groups E and EG were respectively subdivided into two groups which were sacrificed 36 hours(E1,EG1,n = 8)and 7 days(E2,EG2,n = 8)after the last training.All groups except the C un...