红景天、丹参及荷花粉对光气损伤的治疗作用

目的：研究红景天、丹参及荷花粉对光气损伤小鼠的治疗作用。方法：50只小鼠随机分为红景天、丹参、荷花粉治疗组,阳性对照组和阴性对照组,共5组。阴性对照组以空气为对照,其余各组小鼠给予11.9 mg/L的光气1 min,于染毒后20min,各治疗组小鼠分别以0.01ml/g的红景天、丹参和荷花粉灌胃,阳性对照组给予等体积生理盐水,4 h后解剖小鼠取样,测定支气管肺泡灌洗液中蛋白含量、肺脏MDA及GSH含量及进行血细胞测定。结果：红景天治疗组小鼠支气管肺泡灌洗液中蛋白含量显著低于阳性对照组（P〈0.05）;红景天和丹参治疗组小鼠全血血小板较阳性对照组显著升高（P〈0.05）;各治疗组小鼠肺脏MDA含量均较阳性对照组降低,其中红景天组最为显著（P〈0.05）;各治疗组小鼠肺脏GSH含量均较阳性对照组升高,其中红景天组最为显著（P〈0.05）。结论：红景天、丹参和荷花粉均对光气导致的肺损伤具有一定的治疗作用,红景天的治疗作用优于丹参和荷花粉。

Effects of Integripetal rhodiola herb, Salvia miltiorrhiza and lotus flower pollen on acute lung injury induced by phosgene

OBJECTIVE:To study treatment effects of Integripetal rhodiola herb,Salvia miltiorrhiza and lotus flower pollen on injuries induced by phosgene in mice.METHODS:50 mice were randomly divided into 5 groups including Integripetal rhodiola herb group,Salvia miltiorrhiza group,lotus flower pollen group,positive control group and negative control group.The mice in negative control group were exposed to room air and the mice in other groups were exposed to 11.9 mg/L phosgene for 1 minute.After 20 minutes,mice in treatment groups were treated with integripetal rhodiola herb,Salvia miltiorrhiza and lotus flower pollen by 0.1 ml/10g intragastric administration.Mice were sacrificed after 4 hour.The protein content in bronchoalveolar lavage fluid(BLAF),the content of malondialdehyde(MDA) and reduced glutathione(GSH) and blood cell count were determined.RESULTS:The protein content of BLAF in integripetal rhodiola herb group decreased significantly compared with that of positive control(P<0.05).Peripheral blood platelet count increased significantly in mice treated with integripetal rhodiola herb and Salvia miltiorrhiza compared with positive control without treatment(P<0.05).MDA content decreased in three treatment groups and was significantly decreased in Integripetal rhodiola herb compared with positive control group(P<0.05).GSH content increased in three treatment groups and was increased significantly in Integripetal rhodiola herb group compared with positive control(P<0.05). CONCLUSION:Integripetal rhodiola herb,Salvia miltiorrhiza and lotus flower pollen exerted a therapeutic role on lung damage induced by phosgene in mice.Integripetal rhodiola herb efficacy was better than that of Salvia miltiorrhiza and lotus flower pollen.