自制HYD液低温保存大鼠肝脏方法的改进

为探讨改进大鼠肝脏低渐保存方法对鼠肝的影响,即将切取后的鼠肝灌入一定量的自制保存液（ＨＹＤ）后结扎进出肝脏的各血管,应用大鼠离体肝灌注模型,地传统保存法（对照组）和改进保存法（２０％组,３０％组及４０％组）肝脏微循环指标（门静脉灌注压,流出液内内皮素－１,台盼蓝分布时间及组织学改变）,流出液有关酶指标水平及分泌胆汁量进行了系统,结果：２０％组,３０％组及４０％组肝脏微循环指标及分泌胆汁量明显优于对

STUDY ON MODIFIED COLD STORAGE METHOD OF RAT LIVERS WITH SELF-MADE HYD SOLUTION

To investigate the effect of modified cold preservation on rat livers. The modified method was that the vascular bed of rat liver was infused with an additional 20 ml, 30 ml and 40 ml selfmade HYD solution/100g liver. Via portal vein and the vesselses of liver were ligated. Forty rats were randomly divided into four groups including conventional storage group (control group) and modified preservation groups (20%, 30% and 40% group). The indices of hepatic microcirculation, enzymes level in the effluent and bile production were determined in the groups. Results: Bile production and all the indices of hepatic microcirculation including portal perfused pressure, endothelin1 in the effluent, Trypan Blue distrubution time and histology in experimental groups were significantly superior to those in control group (P<0.05). The liver enzymes in 30% group were markedly lower than those in control group (P<0.05). The preservation effect of rat livers in 30% group was the best among the experimental groups. Conclusions: The modified cold storage method is effective and may have potential for clinical application for liver preservation.