| 抗狂犬病疫苗单克隆抗体的制备和初步鉴定 |
| |
| 引用本文： | 赵蓉,游云.抗狂犬病疫苗单克隆抗体的制备和初步鉴定[J].福建医科大学学报,1989,23(3):210-211. |
| |
| 作者姓名： | 赵蓉 游云 |
| |
| 作者单位： | 福建医学院肿瘤研究室，福建医学院肿瘤研究室，福建医学院肿瘤研究室，福建医学院肿瘤研究室 |
| |
| 摘 要： | 用狂犬病疫苗为抗原,免疫BALB/c小鼠,取免疫小鼠脾细胞与小鼠骨髓瘤细胞株SP2/O融合,经过培养、克隆、ELISA鉴定,筛出3株分泌抗狂犬病疫苗单克隆抗体细胞株。双扩散结果证明:3株细胞均分泌IgG1。
|
| 关 键 词： | 单克隆抗体 狂犬病疫苗 酶联免疫吸附试验(ELISA) |
Preparation and Identification on Monoclonal Antibodies Against Rabies Vaccine |
| |
| Zhao Rong,.Preparation and Identification on Monoclonal Antibodies Against Rabies Vaccine[J].Journal of Fujian Medical University,1989,23(3):210-211. |
| |
| Authors: | Zhao Rong |
| |
| Abstract: | The spleen cells from BALB/c mice immunized with rabies vaccine (RV) were fused with SP2/O myeloma cells. Three hybridoma cells line (RV-F_8, RV-D_3, RV-D_(11), ) McAb against RV was obtained after cloning for over two time, its specificity was ide ntified by ELISA. The McAb was identilied as mouse IgG_1. |
| |
| Keywords: | monoclonal antibody Rabies vaccine ELISA |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
