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| 用生物发光法监测RBC—ATP含量及血液保存期RBC—ATP值的变化 | |
| 引用本文： | 李玉奎 丛玉隆. 用生物发光法监测RBC—ATP含量及血液保存期RBC—ATP值的变化[J]. 军医进修学院学报, 1991, 12(1): 54-56 |
| 作者姓名： | 李玉奎 丛玉隆 |
| 作者单位： | 中国人民解放军军医进修学院基础医学研究所，中国人民解放军军医进修学院基础医学研究所，中国人民解放军军医进修学院基础医学研究所，中国人民解放军军医进修学院临床检验科，中国人民解放军军医进修学院输血科 |
| 摘 要： | 以RBC-ATP含量作为血液保存质量的监测在国外已应用多年。我们用虫发光素酶生物发光法测定健康成年人32例RBC-ATP含量,以及血液保存期间RBC-ATP含量的变化,发现血液保存时间愈长,RBC-ATP含量下降愈显著。我们认为虫发光素酶生物发光法测定RBC-ATP含量,方法简便快速,准确可靠,实为血液保存质量监测的最佳选择。 |
| 关 键 词： | 红细胞 腺苷三磷酸 萤虫素酶类 血液保存 |
ATP level in red blood cells and its alteration during storage determined by the fire-fly luciferase method |
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| LI Yu-kui,et al. ATP level in red blood cells and its alteration during storage determined by the fire-fly luciferase method[J]. Academic Journal of Pla Postgraduate Medical School, 1991, 12(1): 54-56 | |
| Authors: | LI Yu-kui et al |
| Abstract: | The content of ATP in red blood cells has been used as a measure of viability for the evaluaation of storage of blood abroad for several years. At home we completed the determination of ATP in red cells in 32 adult healthy people, using a simple procedure of the fire-fly lueiferase method, we also observed the change of ATP content in red cells during storage. The results showed that the ATP level in red cells decreased, the longer the time the more the decrease. It is suggested that the fire-fly luciferase ATP assay is a simple, rapid, accurate, and reliable method, as a measure of viability, for the evaluation of storage red cells. |
| Keywords: | Erythrocytes Adenosine triphosphate Luciferase Blood preservation Quality control |
| 本文献已被 CNKI 维普 等数据库收录！ | |