原因不明性肝炎患者血清中TTV DNA的检出及其临床意义

目的：研究原因不明性肝炎患者输血传播性病毒（Rransfusion transmitted virus,TTV)感染的检出状况及其临床意义。方法：在TTV ORF1保守区设计引物，建立巢式聚合酶链反应（Nested-PCR),检测50例原因不明性肝炎、40例健康人与30例乙肝病毒携带者血清中TTV DNA，比较TTV DNA阳性的肝炎患者的临床特征。结果：50例原因不明性肝炎中16例TTV DNA呈阳性（占32％），比健康人群（2／40，5％）与乙肝病毒携带者（2／30，6．7％）血清中TTV DNA检出率高（P＜0．05），16例TTV DNA阳性患者中以慢性肝炎占多数（12／16，75．5％），临床表现为反复、持续ALT异常。结论：TTV在原因不明性肝炎中感染率明显高于其他人群，其可能是本地区原因不明性肝炎的致病因子。

A study of TTV DNA detection and clinical significance in patients with hepatitis of unknown etiology

Objective:To investigate the presence of TTV infection among patients with hepatitis of unknown etiology and its clinical significance.Methods:A nested polymerase chain reaction(PCR) assay with primers from ORF1 of TTV genome was established to detect TTV DNA in 50 patients with hepatitis of unknown etiology,in 40 health persons and 30 HBV carriers.Results:The positive rate of TTV DNA accounted for 32%(16/50) in patients with hepatitis of unknown etiology,compared with the positive rate of TTV DNA accounting for 5%(2/40) in health persons and 6.7%(2/30) in HBV carriers. It had statistical difference(P<0.05). Among 16 patients with TTV DNA positive response,12 cases were chronic hepatitis(12/16,75%).Conclusion:TTV infection will be one of the major causes of hepatitis of unknown etiology in this area.