氯化锂对人眼Tenon囊成纤维细胞增殖的影响 |
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引用本文: | 王继兵,黄旭东,刘姗姗,满辉.氯化锂对人眼Tenon囊成纤维细胞增殖的影响[J].美中国际眼科杂志,2012(7):1237-1241. |
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作者姓名: | 王继兵 黄旭东 刘姗姗 满辉 |
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作者单位: | [1]潍坊医学院眼科学,中国山东省潍坊市261041 [2]潍坊眼科医院青光眼学组,中国山东省潍坊市261042 |
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基金项目: | 山东省自然科学基金资助项目(No.ZR2010HM015) |
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摘 要: | 目的:观察氯化锂(Lithium chloride)对人眼Tenon囊成纤维细胞(human Tenons capsule fibroblasts, HTFs)体外生长的抑制效应,并探讨其作用机制。 方法:磺基罗丹明(SRB)法和BrdU法检测细胞生长抑制率;采用流式细胞技术(FCM)检测细胞凋亡和细胞周期的变化;Hoechst 33258染色后荧光显微镜观察细胞形态学的改变。结果:在40-160mmol/L范围内,氯化锂能够抑制HTFs的增殖,且呈剂量与时间依赖性。氯化锂使HTFs阻滞于G2/M期,且能诱导细胞凋亡,高浓度组尤为明显。结论:氯化锂能抑制HTFs增殖,机制为诱导细胞凋亡,并将细胞阻滞于G2/M期。
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关 键 词: | 氯化锂 人眼Tenon囊 成纤维细胞 生长抑制 |
Inhibitory effects of Lithium chloride on human Tenon's capsule fibroblasts |
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Authors: | Hui-Hui Zou Ji-Bing Wang Xu-Dong Huang Shan-Shan Liu Hui Man |
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Institution: | 1Weifang Medical University, Ophthalmology, Weifang 261041, Shandong Province, China; 2Weifang Eye Hospital, Weifang 261042, Shandong Province, China |
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Abstract: | AIM: To investigate the inhibitory effect of Lithium chloride on human Tenons capsule fibroblasts(HTFs) and explore its mechanism. METHODS: The SRB and BrdU methods were used to observe the activity of HTFs after treated with Lithium chloride in vitro, apoptosis percentage and cell cycle were analyzed by flow cytometry (FCM).Morphological changes in apoptotic cells were observed by Hoechst 33258 fluorescence staining.RESULTS: The cultured HTFs were significantly inhibited by Lithium chloride in concentration of 40, 80, 160mmol/L compared with the control group and it was dose-dependent and time-dependent.It was demonstrated that Lithium chloride could evoke G2/M cell cycle arrest and induce apoptosis,especially in the high concentration groups. CONCLUSION: Lithium chloride could restrain growth of HTFs by evoke G2/M cell cycle arrest and induce apoptosis. |
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Keywords: | Lithium chloride human Tenon'scapsule fibroblasts growth inhibition |
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