新型呼肠病毒M片段对核转录因子κB的活性抑制的研究

目的：探讨新型呼肠病毒M片段能否抑制核转录因子-κB（nuclear factor kappa-light-chain-enhancer of activated B cells, NF-κB）的活性。方法构建3个不同中基因节段的重组真核表达质粒，并瞬时转染真核细胞，利用荧光素酶双报告系统检测其对NF-κB激活有抑制效应的基因。结果酶切鉴定3个基因重组质粒均构建成功。其中M3基因表达的蛋白对肿瘤坏死因子α介导的NF-κB激活有较明显的抑制，抑制率达55%。而M1和M2基因表达的蛋白较之于空载体均无明显的抑制效果。结论 M3基因能显著抑制肿瘤坏死因子α介导的NF-κB激活，为研究病毒的致病及免疫调控机制提供了重要理论依据。

Inhibition of the activation of nuclear factor kappa-light-chain-enhancer of activated B cells by M fragments of new type of reovirus

Objective To investigate the effects of M fragments of new type of reovirus on the inhibition of the activation of nuclear factor kappa-light-chain-enhancer of activated B cells （NF-κB）. Methods 293T cells were transfected with different recom-binant plasmids containing M1, M2 and M3 fragments respectively. Then the dual luciferase reporter system was applied to identify the effects of different genes on the inhibition of NF-κB activation. Results Three recombinant plasmids were all constructed suc-cessfully. M3 gene could inhibit tumor necrosis factor （TNF）-α-induced NF-κB activation significantly （55%reduction was detected）. While M1 and M2 genes had no significant inhibitory effect on NF-κB activation. Conclusions M3 gene can inhibit TNF-α-indu-ced NF-κB activation significantly, which provides an evidence for the studies on viral pathogenesis and immunomodulatory properties.