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| 无精症患者Y染色体微缺失的多重PCR筛查 | |
| 引用本文： | 于丛一,庄广伦,周灿权,闫宗合,李巍,高华,芮德蓉. 无精症患者Y染色体微缺失的多重PCR筛查[J]. 中国男科学杂志, 2003, 17(6): 369-372 |
| 作者姓名： | 于丛一 庄广伦 周灿权 闫宗合 李巍 高华 芮德蓉 |
| 作者单位： | 1. 中山大学附属第二医院生殖中心,广州,510120 2. 中山大学附属第一医院生殖中心 3. 中山大学达安基因诊断中心 |
| 摘 要： | 目的 建立一套Y染色体微缺失的多重PCR筛查方法。方法 建立5套稳定和可靠的多重PCR筛查方法，对进行ICSI治疗的26例无精子症患者和进行睾丸活检的30例无精子症患者进行Y染色体微缺失的检测。结果 在56例无精子症患者中发现5例AZFc／DAZ缺失，2例同时具有AZFc／DAZ和AZFb／RBMl的缺失，1例仅具有sY153一个片段的缺失。结论 本研究Y染色体微缺失的多重PCR筛查方法是易行和可靠的，对无精子症患者有必要进行Y染色体微缺失的常规筛查。 |
| 关 键 词： | Y染色体微缺失 多重PCR 男性不育 无精症 染色体 |
| 修稿时间： | 2003-06-03 |
Multiplex PCR for screening of microdeletions on the Y chromosome in azoospermic patients |
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| Yu Congyi,Zhuang Guanglun,Zhou Canquan,Yan Zonghe,Li Wei,Gao Hua,Rui DerongReproductive Medical Center. Multiplex PCR for screening of microdeletions on the Y chromosome in azoospermic patients[J]. Chinese Journal of Andrology, 2003, 17(6): 369-372 | |
| Authors: | Yu Congyi Zhuang Guanglun Zhou Canquan Yan Zonghe Li Wei Gao Hua Rui DerongReproductive Medical Center |
| Affiliation: | Yu Congyi,Zhuang Guanglun,Zhou Canquan,Yan Zonghe,Li Wei,Gao Hua,Rui DerongReproductive Medical Center,Department of Obstetrics and Gynecology,the Second AffiliatedHospital of Sun Yatsen University,Guangzhou 510120,China |
| Abstract: | Objective To develop a multiplex PCR protocol for routine screening of microdeletions on the Y chromosome. Methods Five multiplex sets were established. 26 azoospermic men undergoing ICSI and 30 azoospermic men undergoing testicular biopsy were screened for microdeletions of Y chromosome. Results In 56 azoospermic men, it was founded 5 patients with AZFc/DAZ microdeletions, 2 patients with AZFc/DAZ and AZFb/RBMl microdeletions, and 1 patient with single sY153 microde-letion. Conclusion The multiplex PCR protocol presented in this study is an easy and reliable method for detection of microdeletions on the Y chromosome. Routine screening of microdeletions on the Y chromosome for azoospermic patients is essential. |
| Keywords: | Y chromosome microdeletions multiplex PCR male infertility |
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