超声波对小鼠胚泡着床影响的研究

本实验证明频率800kHz,声强1.75W/cm~2连续超声波在着床前单纯照射小鼠子宫部一次(一分钟),可显著地抑制着床,二次照射可完全阻遏着床。用放射免疫法测定小鼠着床前外周血中雌二醇和孕酮含量,结果表明照射组与对照组间无显著性差异。提示抗着床作用与这两种激素在外周血中的浓度无关。光镜、电镜下观察到的子宫内膜形态学变化为大量白细胞浸润;内膜腔上皮微绒毛减少、消失;糖蛋白衣过早地被清除;膜性结构线粒体及内质网的损害等,这些形态学变化可能是影响着床的重要因素。

AN EXPERIMENTAL STUDY OF THE EFFECT OF ULTRASOUND ON THE NIDATION OF MOUSE BLASTOCYST

The consecutive ultrasonic wave with frequency of 800 KHz and sound intensity of1.75w/cm was used to irradiate mouse uterus before nidation in this experiment.Therate of nidation in experimental groups with an irradiation for a minutc was 18.75%,the rate of nidation in control groups was 62.96%.There was a significant correlation betweenthem(P<0.005).The nidation could be totally checked after two irradiations of ultrasonicwave.The amount of estradiol and progesterone in peripheral blood was determined byradiommunoassay(RIA)in mouse before nidation.The rcsults show that there was nosignificant difference between the experimental groups with irradiation of ultrasounic waveand the control groups.This indicates that the anti-nidation effect was irrelevant to theconcentration of those two hormones(estradiol and progesterone)in peripheral blood.The morphologic changes in the endometrium were observed under optical and electronmicroscope.There were many leucocytes infiltrated into the endometrium,the microvillidecreased and disappeared.The impairment of mitochondric and endoplasmic reticulummight be the main factors influencing nidation.