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| 两种方法检测血清甲胎蛋白(AFP)的比较 | |
| 引用本文： | 邝妙欢,林翠杏,刘万里,欧阳文婷,姚少羽,邱瑞芬,钟义富.两种方法检测血清甲胎蛋白(AFP)的比较[J].国际医药卫生导报,2003,9(22):9-10. |
| 作者姓名： | 邝妙欢 林翠杏 刘万里 欧阳文婷 姚少羽 邱瑞芬 钟义富 |
| 作者单位： | 1. 中山大学肿瘤医院检验科,广东,广州,510060 2. 中山大学肿瘤医院放疗科,广东,广州,510060 3. 中山大学附属第一医院检验科,广东,广州,510080 |
| 摘 要： | 目的对电化学发光免疫法与微粒子酶联免疫法检测血清甲胎蛋白作对比分析.方法随机抽取150份临床送检的标本,用电化学发光免疫法与微粒子酶联免疫法测试甲胎蛋白的含量,对测试数据进行相关性试验、线性试验、精密度试验、回收试验.结果两法无显著性差异(P＞0.05),但电化学发光免疫法的线性范围较宽(1-980ng/ml),重复性较好(批内CV值3.4%,批间CV值3.5%),回收率较高(97.2%).结论电化学发光免疫法检测血清甲胎蛋白优于微粒子酶联免疫法检测血清甲胎蛋白 |
| 关 键 词： | 电化学发光免疫法 微粒子酶联免疫法 甲胎蛋白 |
| 文章编号： | 1007-1245(2003)011-0009-02 |
| 修稿时间： | 2003年9月20日 |
Comparison of Two Methods for Detection of the Levels of Serum AFP |
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| Kuang miaohuan,Lin cuixing,Liu wanli,et al.Comparison of Two Methods for Detection of the Levels of Serum AFP[J].International Medicine & Health Guidance News,2003,9(22):9-10. | |
| Authors: | Kuang miaohuan Lin cuixing Liu wanli |
| Institution: | Kuang miaohuan,Lin cuixing,Liu wanli,et alDepartment of laboratory,Cancer Center,Sun Yat-Sen university,Guangzhuo 510060 |
| Abstract: | Objective To evaluate the efficiencies of electrochemiluminescent assay (ECL) andmicroparticle enzyme immunoassy(MEIA)for detecting of the levels of serum AFP .Methods Serum AFPlevels of 150 samples were determined with both ECL and MEIA. The correlation between ECL and MEIA wereassessed, and the assays of linear range, recovery and precision of the two methods were performed andalso evaluated.Results There was no significant difference between ECL and MEIA for detection of thelevels of serum AFP (P>0.05). Overall, however, in comparison with MEIA, ECL had a broader linear range(1-980ng/ml) and a higher precision (the within-run, 3.4%; the between-run, 3.5%) as well as a higheraverage recovery rate (97.2%).Conclusions There was a good correlation between ECL and MEIA fordetermination of the levels of serum AFP. In general, for detecting the levels of serum AFP, ECL wassuperior to MEIA. |
| Keywords: | ECL MEIA AFP |
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