甲硝唑对阴道毛滴虫作用的流式细胞分析

目的 :了解甲硝唑对阴道毛滴虫的作用机制。方法 :应用流式细胞术 ( FCM)检测阴道毛滴虫的细胞周期和甲硝唑对该虫细胞周期的影响。结果 :对照组中 ,阴道毛滴虫细胞周期的时相分布为 G0 /G1期 70 .3%,S期 13.6% ,G2 M期 13.5%和自然死亡率为 2 .8%左右。各用药组虫体的细胞周期时相因药物浓度和随时间推移而改变。尤其给药 9h后 ( 2 0 μg/ml) ,虫体死亡率为4 6.6% ,与对照组相比有显著性差异 ( P<0 .0 1),G0 /G1期和 G2 M期虫体明显减少 ,S期出现积累。结论 :甲硝唑对阴道毛滴虫 G2 M期有抑制作用。

FLOW CYTOMETRIC ANALYSISOF THE EFFECT OF METRONIDAZOLE ON TRICHOMONAS VAGINALIS

AIM:To understand the mechanism of action of metronidazole on Trichomonas vaginalis.METHODS:The cell cycle of T.vaginalis and the effect of metronidazole on T.Vaginalis in vitro were determined by flow cytometry. RESULTS:In the control untreated T. vaginalis,the percentages of cells in G0 /G1 ,S and G2 M phases were70 .3% ,1 3.6 % and 1 3.5 % ,respectively in phasic distribution of cell cycle,with a natural mortality of 2 .8% . The phasic distribution of cell cycle of T.vaginalis was obviously changed with the concentration of metronidazole and the time duration of incubation. After 9-hr incubation at 20 μg/ml of metronidazole concentration, the death rate of cell was 46. 6% , being significantly different as compared with the control group (P <0. 01). The G0/G1 and G2M cells were obviously decreased in number, while S cells were accumulated. CONCLUSION:Metronidazole inhibited G2M phase of T. vaginalis.