| 日本血吸虫 NADH脱氢酶 1和细胞色素 C氧化酶 1基因部分序列 |
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| 引用本文： | 邱持平,张立华,E.Sorensen,D.P.McManus.日本血吸虫 NADH脱氢酶 1和细胞色素 C氧化酶 1基因部分序列[J].中国寄生虫学与寄生虫病杂志,1997,15(6):378-381. |
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| 作者姓名： | 邱持平 张立华 E.Sorensen D.P.McManus |
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| 作者单位： | 1 中国预防医学科学院寄生虫病研究所;
2 Molecular Helminthology Laboratory, Tropical Health
Program, The Queensland Institute of Medical Research, Australia |
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| 摘 要： | 目的 :测定和比较日本血吸虫浙江株和安徽株 NADH脱氢酶 1( NADH1)和细胞色素 C氧化酶 1( Cytc1)基因部分序列。方法:用 PCR方法从特定的引物扩增出 NADH1和 Cytc1DNA基因 ,然后进行测序。结果 :获得两株日本血吸虫成虫 NADH1和 Cytc1基因及其序列。结论 :两株日本血吸虫成虫 NADH1基因及其部分序列除第 382位核苷酸不同 ( C/T)外 ,其余的均相同。两株日本血吸虫成虫Cytc1基因及其部分序列完全相同。
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| 关 键 词： | 日本血吸虫 NADH脱氢酶1 细胞色素C氧化酶1 PCR DNA序列 |
PARTIAL SEQUENCE OF NADH DEHYDROGENASE 1 AND CYTOCHROME C OXIDASE 1 GENE OF SCHISTOSOMA JAPONICUM |
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| Qiu Chiping,Zhang Lihua,E.Sorensen,D.P.Mc Manus.PARTIAL SEQUENCE OF NADH DEHYDROGENASE 1 AND CYTOCHROME C OXIDASE 1 GENE OF SCHISTOSOMA JAPONICUM[J].Chinese Journal of Parasitology and Parasitic Diseases,1997,15(6):378-381. |
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| Authors: | Qiu Chiping Zhang Lihua ESorensen DPMc Manus |
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| Institution: | 1 Institute of Parasitic Diseases, Chinese Academy of Prevantive Medicine, Shanghai 200025
2 Molecular Helminthology Laboratory, Tropical Health Program, The Queensland Institute of Medical Research, Australia |
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| Abstract: | AIM:To determine and compare NADH dehydrogenase1( NADH1) and cytochrome C oxidase1( Cytc1) partial gene sequences of Zhejiang and Anhui isolates of Schistosoma japonicum. METHODS: NADH1 and Cytc1 genes were amplified by PCR using special primers corresponding with 44 1and 370 bp of the genomic DNAs, and subsequently sequenced. RESULTS:NADH1 and Cytc1 gene sequences were obtained from the two isolates. CONCLUSION: The partial sequences of the NADH1 genes were essentially identical for both isolates with only one nucleotide difference (C/T ) at position 382. The Cytc1 sequences were virtually identical for the two isolates. |
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| Keywords: | Schistosom a japonicum NADH dehydrogenase1 cytochrome C oxidase1 PCR sequence |
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